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ProteomicsE3 ligase substrate adaptor SPOP fine tunes the UPR of pancreatic beta cells
The Cullin 3 E3 ligase adaptor protein, SPOP, targets proteins for ubiquitination and proteasomal degradation. We previously established the β cell transcription factor (TF) and human diabetes gene PDX1 as an SPOP substrate, suggesting a functional role for SPOP in the β cell. Here, we generated a β...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-12 | PXD052527 | Pride
E3 ligase Pdx1 Spop Proteasomal degradation Diabetes 
TranscriptomicsTranscription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1
In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expre...
ORGANISM(S): Mus musculus 
2010-01-01 | E-MTAB-132 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of the islets from Pdx1+/- mice compared to islets isolated from Pdx1 +/+ littermates
The aim of this experiment was to use global gene expression profiling to identify genes that are differentially expressed in the islets from Pdx1+/- mice compared to islets isolated from Pdx1 +/+ littermates. The Pdx1 null mutation consists of a nuclear targeted _-galactosidase cassette fused in-fr...
ORGANISM(S): Mus musculus 
2009-12-02 | E-MTAB-127 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of mouse MIN6 pancreatic beta cells to identify Pdx1 targets
The aim of this experiment was to use highthroughput chromatin immunoprecipitation followed by hybridization to promoter microarrays to obtain a comprehensive list of sites in the genome that are physically occupied by Pdx1. Chromatin was prepared from Min6 insulinoma cells and immunoprecipitated w...
ORGANISM(S): Mus musculus 
2009-12-02 | E-MTAB-134 | biostudies-arrayexpress
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