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TranscriptomicsTranscription profiling of mouse islet growth after partial pancreatectomy and exendin-4 Treatment
Diabetes mellitus results from an inadequately functioning beta-cell mass. In the adult pancreas, beta-cell mass is dynamic, increasing to meet metabolic demands and decreasing with metabolic or injury insults. Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor agonist that augments beta-cell m...
ORGANISM(S): Mus musculus 
2005-12-01 | E-CBIL-5 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of mouse pancreatic beta cells of MIN6 insulinoma cell line transfected with siRNA directed against murine Meis3 or non targeting control.
In order to identify genes that are regulated by Meis3, MIN6 insulinoma cells were transfected with siRNA directed against murine Meis3 (siMeis3) or non targeting control (siControl). RNA was harvested and reverse transcribed. Reduced expression of Meis3 was confirmed. The RNA was then submitted for...
ORGANISM(S): Mus musculus 
2010-11-01 | E-MTAB-273 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1
In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expre...
ORGANISM(S): Mus musculus 
2010-01-01 | E-MTAB-132 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of the islets from Pdx1+/- mice compared to islets isolated from Pdx1 +/+ littermates
The aim of this experiment was to use global gene expression profiling to identify genes that are differentially expressed in the islets from Pdx1+/- mice compared to islets isolated from Pdx1 +/+ littermates. The Pdx1 null mutation consists of a nuclear targeted _-galactosidase cassette fused in-fr...
ORGANISM(S): Mus musculus 
2009-12-02 | E-MTAB-127 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of mouse MIN6 pancreatic beta cells to identify Pdx1 targets
The aim of this experiment was to use highthroughput chromatin immunoprecipitation followed by hybridization to promoter microarrays to obtain a comprehensive list of sites in the genome that are physically occupied by Pdx1. Chromatin was prepared from Min6 insulinoma cells and immunoprecipitated w...
ORGANISM(S): Mus musculus 
2009-12-02 | E-MTAB-134 | biostudies-arrayexpress
TranscriptomicsPdx1 maintains ?-cell identity and function by repressing an ?-cell program
To get a more complete picture of the transcriptional changes associated with Pdx1 loss in ?-cells, we conducted an mRNA microarray comparing normal islet ?-cells and a-cells to the reprogrammed cells from PKO mice. Islet beta cells are from mice which has a single copy of Pdx1 flox (Pdx1L/+) allele...
ORGANISM(S): Mus musculus 
2013-11-19 | E-GEOD-52258 | biostudies-arrayexpress
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