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ProteomicsHigh Field Asymmetric Waveform Ion Mobility Spectrometry- Mass Spectrometry to Enhance Cardiac Muscle Proteome Coverage
Heart tissue sample preparation for mass spectrometry (MS) analysis that includes pre-fractionation reduces the cellular protein dynamic range and increases the relative abundance of non-sarcomeric proteins. We previously described “IN-Sequence” (IN-Seq) where heart tissue lysate is sequentially par...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-20 | PXD039174 | Pride
Faims Dia-ms Cardiac In-seq 
ProteomicsHuman limbal epithelial cell-derived exosomes cargo profile and their regulatory roles in limbal stromal cells in diabetic and non-diabetic cornea
Limbal stem cells including epithelial and stromal/Mesenchymal stem cells that contribute to sustained corneal homeostasis, maintain their ability to act as self-renewal progenitor cells by virtue of their limbal niche and intercellular communication. Extracellular vehicles (EVs), including exosomes...
ORGANISM(S): Homo sapiens (Human) 
2023-10-23 | PXD040918 | Pride
Diabetes Cornea Exosomes 
ProteomicsFunctional Assessment of US SARS-CoV-2 L452R Epsilon Variant
The multiple mutations comprising the epsilon variant demonstrates the independent convergent evolution of SARS-CoV-2 with its spike protein mutation L452R also present in the delta variant. Cells infected with live viral samples of the epsilon viral variant compared to non-epsilon variant displa...
ORGANISM(S): Chlorocebus sabaeus 
2022-05-06 | PXD027995 | Pride
Faims Covid19 Vero 
ProteomicsMitoPlex: A Targeted Multiple Reaction Monitoring Assay for Quantification of a Curated Set of Mitochondrial Proteins
Mitochondria are the major source of cellular energy (ATP), as well as critical mediators of widespread functions such as cellular redox balance, apoptosis, and metabolic flux. Methods to quantify mitochondrial content are limited to low throughput immunoassays, measurement of mitochondrial DNA, or ...
ORGANISM(S): Mus Musculus 
2020-04-09 | PXD017884 | panorama
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