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TranscriptomicsLigand-Dependent Enhancer Activation Regulated by Topoisomerase-I Activity
The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stress f...
ORGANISM(S): Homo sapiens 
2015-01-22 | E-GEOD-63202 | biostudies-arrayexpress
TranscriptomicsGlobal Analysis of the developmental dynamics of Gossypium hirsutum based on strand-specific transcriptome
Cotton is the most important economic crop that provides natural fibre and by-products such as oil and protein. The global gene expression could provide insight into the biological processes underlying growth and development, which involving suites of genes expressed with temporal and spatial contro...
ORGANISM(S): Gossypium hirsutum 
2016-02-29 | E-GEOD-70369 | biostudies-arrayexpress
TranscriptomicsUbe3a-ATS characterization with strand-speicific microarray
This strand-specific array is performed to characterize expression features of Ube3a-ATS, including its imprinting status, its exon-intron structure, its transcriptional initiation and termination site as well as its polyadenylation status. The array contains reverse-complementary probes detecting t...
ORGANISM(S): Mus musculus 
2012-05-02 | E-GEOD-29254 | biostudies-arrayexpress
GenomicsLong-range joining of intra-chromosomal DNA double-strand breaks
Antibody class switch recombination (CSR) in B lymphocytes joins two DNA double-strand breaks (DSBs) lying 100 to 200 kilobases (kb) apart within switch (S) regions in the immunoglobulin heavy chain locus (IgH). CSR-activated B lymphocytes generate multiple S-region DSBs in the donor Sm and in a do...
ORGANISM(S): Mus musculus 
2014-01-03 | E-GEOD-53755 | biostudies-arrayexpress
TranscriptomicsXanthomonas campestris pv. Campestris 8004 far-red light irradiation and XccBphP overexpression transcriptional analysis
RNA isolation and RNA-Seq: Wild-type Xcc or pXccBphP strains were cultured in far-red light or dark conditions up to logarithmic phase (0.7 to 0.8 OD600) at 28C in PYM broth. Total bacterial RNA was isolated using the MasterPureTM RNA Purification Kit (Epicentre, Illumina). Samples corresponding to ...
ORGANISM(S): Xanthomonas campestris pv. campestris 
2016-12-03 | E-MTAB-4958 | biostudies-arrayexpress
TranscriptomicsCharacterization of the Imprinting Signature of Mouse Embryo Fibroblasts by RNA Deep Sequencing
Mouse embryo fibroblasts (MEFs) closely resemble mouse embryos and are convenient sources for biochemical studies when cell number may be limiting from mouse embryos. To derive the imprinting signature of MEFs and potentially detect novel imprinted genes we characterized them using strand- and allel...
ORGANISM(S): Mus musculus 
2013-11-18 | E-GEOD-49538 | biostudies-arrayexpress
TranscriptomicsDefault DNA Methylation is Preceded by Broad, Low-Level Transcription in Fetal Male Germ Cells and Is Inversely Patterned by Dynamic H3K4 Methylation (ChIP-chip and MIRA-chip)
To understand what dictates the emerging patterns of de novo DNA methylation, we mapped DNA methylation, chromatin, and transcription changes in purified fetal mouse germ cells using MIRA-chip, ChIP-chip, and strand-specific RNA-seq, respectively. De novo methylation occurred without any apparent tr...
ORGANISM(S): Mus musculus 
2013-07-31 | E-GEOD-45836 | biostudies-arrayexpress
TranscriptomicsDegradome sequencing reveals endogenous small RNA targets in rice (Oryza sativa L. ssp. indica)
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) regulate gene expression in eukaryotes. Plant miRNAs modulate their targets mainly via messenger RNA (mRNA) cleavage. Small RNA targets have been extensively investigated in Arabidopsis using computational prediction, experimental validation, an...
ORGANISM(S): Oryza sativa Indica Group 
2010-01-01 | E-GEOD-19050 | biostudies-arrayexpress
GenomicGermline De Novo Mutations with Parent of Origin in WGS Trios

This study is part of the 'First 1,000 Days of Life and Beyond' study at the Inova Translational Medicine Institute. Whole-genome sequencing data from 1,291 parent-offspring trios was used to study the properties of clustered de novo mutations. The maternal clusters were fo...

phs001522 | dbGaP
UnknownMismatch cleavage by single-strand specific nucleases.
Not available
S-EPMC419476 | biostudies-literature
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