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ProteomicsMannosidic glycans on misfolded Arabidopsis SUBEX glycopeptides
The mutated extracellular domain of Arabidopsis STRUBBELIG (SUBEX) was fused to GFP and either transiently expressed in Nicotiana benthamiana leaves or stably in Arabidopsis thaliana seedlings and purified. The purified SUBEX proteins were subjected to SDS-PAGE and CBB staining. Corresponding gel pi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Nicotiana benthamiana 
2025-10-13 | PXD066844 | Pride
Endoplasmic reticulum‐associated protein degradation (erad) Endoplasmic reticulum (er) Cell biology Glycoprotein N-linked glycosylation Protein degradation Plant 
ProteomicsImpact of mutations on the plant-based production of recombinant SARS-CoV-2 RBD
Recombinant RBD variants from SARS-CoV-2 spike were produced by transient expression in Nicotiana benthamiana plants and purified. The purified proteins were digested with proteases and glycopeptides were analysed by MS to identify the N-glycan structures.
ORGANISM(S): Nicotiana benthamiana 
2024-01-26 | PXD044462 | Pride
Glycosylation Virus Glycoprotein Vaccine Antigen Nicotiana benthamiana Spike protein 
ProteomicsImplications of O-glycan modifications in the hinge region of a plant-produced SARS-CoV-2-IgA antibody on functionality
In this study, we carried out host engineering to generate a therapeutic glycoprotein largely devoid of plant-endogenous O-glycans for functional characterization. We generated four variants of a potentSARS-CoV-2 neutralizing antibody, COVA2-15 IgA1. The variants that differed in the number of modif...
ORGANISM(S): Nicotiana benthamiana 
2024-02-27 | PXD050139 | Pride
Glycosylation Antibody Glycoprotein Nicotiana benthamiana Virus 
ProteomicsIn planta deglycosylation improves the SARS-CoV-2 neutralization activity of recombinant ACE2-Fc
The ectodomain of human ACE2-Fc was transiently expressed in leaves of Nicotiana benthamiana plants and protein A and SEC purified. SEC-purified ACE2-Fc was sequentially digested with chymotrypsin and trypsin and the glycopeptides were subjected to LC-ESI-MS analysis using the LC-OTMS, Orbitrap Expl...
ORGANISM(S): Nicotiana benthamiana 
2023-04-18 | PXD041320 | Pride
Recombinant protein Glycosylation Nicotiana benthamiana Glycoprotein 
UnknownUnraveling the synergistic effects of Cu-Ag tandem catalysts during electrochemical CO2 reduction using nanofocused X-ray probes.
Not available
S-EPMC10687089 | biostudies-literature
UnknownTwo phase 3 trials of bapineuzumab in mild-to-moderate Alzheimer's disease.
Not available
S-EPMC4159618 | biostudies-literature
UnknownCharacterizing Long COVID Symptoms During Early Childhood.
Not available
S-EPMC12117493 | biostudies-literature
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