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TranscriptomicsTranscription profiling by array of E. coli BL21(DE3)::TN7T7 -SOD to analyze the cellular response of this plasmid-free expression system to high level expression of recombinant human super-oxide-dismutase
This transcription profiling time course experiment was conducted in order to analyze the cellular response of the plasmid-free expression system E.coli BL21(DE3)::TN7 to high level expression of recombinant human super-oxide-dismutase (SOD).Three biological replicates were generated by usi...
ORGANISM(S): Escherichia coli 
2013-05-31 | E-MARS-24 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array for comparison of industrial Escherichia coli strains on growth, transcriptome and proteome level in batch cultivations to identify strain specific features (subexperiment K-12 platform)
The objective of the current study was to evaluate the performance of frequently used E. coli K-12 strains HMS174 and RV308 in the context of industrial cultivations. To meet this objective, we carried out batch cultivations with high-glucose media in computer-controlled bioreactors and applied tran...
ORGANISM(S): Escherichia coli 
2013-08-19 | E-MARS-20 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array for comparison of industrial Escherichia coli strains on growth, transcriptome and proteome level in batch cultivations to identify strain specific features (subexperiment BL21 platform)
The objective of the current study was to evaluate the performance of frequently used E. coli BL21 in the context of industrial cultivations. To meet this objective, we carried out batch cultivations with high-glucose media in computer-controlled bioreactors and applied transcriptome analysis to int...
ORGANISM(S): Escherichia coli 
2013-08-19 | E-MARS-21 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to limited induction with IPTG
This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a limited induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced under w...
ORGANISM(S): Escherichia coli 
2010-03-26 | E-MARS-17 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli BL21(DE3) to analyze the cellular response to high level expression of recombinant human super-oxide-dismutase (SOD)
This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli BL21(DE3) to high level expression of recombinant human super-oxide-dismutase (SOD) on the transcriptional level. Three biological replicates were generated by using a carbon limite...
ORGANISM(S): Escherichia coli 
2011-09-30 | E-MARS-19 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to full induction with IPTG
This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a single pulse induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced un...
ORGANISM(S): Escherichia coli 
2010-03-26 | E-MARS-16 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli HMS174(DE3) to analyze the cellular response to high level export of recombinant protein to the periplasm
This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli HMS174(DE3)-pET30a-sSpAD-GFPmut3.1-Strep to recombinant protein export to the periplasm. Three biological replicates were generated by using a carbon limited exponential fed-batch c...
ORGANISM(S): Escherichia coli 
2013-10-10 | E-MARS-22 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of E. coli HMS174(DE3) to analyze the cellular response to high level cytoplasmic protein expression
This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli HMS174(DE3)-pET30a-GFPmut3.1-Strep to high level cytoplasmic protein expression. Three biological replicates were generated by using a carbon limited exponential fed-batch cultivati...
ORGANISM(S): Escherichia coli 
2013-10-10 | E-MARS-23 | biostudies-arrayexpress
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