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MetabolomicsSubcellular antibiotic visualization reveals a dynamic drug reservoir in infected macrophages
Tuberculosis, caused by the intracellular pathogen Mycobacterium tuberculosis, remains the world's deadliest infectious disease. Sterilizing chemotherapy requires at least 6 months of multidrug therapy. Difficulty visualizing the subcellular localization of antibiotics in infected host cells means t...
2020-12-07 | MTBLS959 | MetaboLights
MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
TranscriptomicsSmall RNAs subcellular distribution in breast cancer and normal cell lines
We detect the small RNAs subcellular distribution in breast cancer cell lines MCF-7 and MDA-MB-231, and normal cell line MCF-10A. Each cell line, we detected the nuclear and cytoplasmic small RNAs expression intensity; and then we could get the nuclear-cytoplasmic-ratio.
ORGANISM(S): Homo sapiens 
2010-05-18 | E-GEOD-15937 | biostudies-arrayexpress
ProteomicsSimultaneous characterisation of RNA and protein subcellular dynamics
Existing methods to analyse RNA localisation are constrained to specific RNAs or subcellular niches, precluding the cell-wide mapping of RNA. We present Localisation of RNA (LoRNA), which maps, at once, RNAs to membranous (nucleus, ER and mitochondria) and membraneless compartments (cytosol, nucleol...
ORGANISM(S): Homo sapiens (Human) 
2022-01-25 | PXD030456 | Pride
Dynamics Subcellular localisation Tmt Spatial-proteome Lopit 
ProteomicsEnrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary (CHO) cells have been widely employed for production of recombinant proteins (RP) for research and therapeutic purposes. Indeed, this expression system was used for production of most of approved antibodies (84%) in 2015-2018 period. The success of this cell line for RP product...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-08-27 | PXD019778 | Pride
Organelles Subcellular fractionation Subcellular proteomics Chinese hamster ovary cells 
ProteomicsA snapshot of Bothrops jararaca snake venom gland subcellular proteome
The synthesis of snake venom proteins is subjected to finely regulated processes in the specialized secretory epithelium within the venom gland. Such processes occur within a defined time frame in the cell and at specific cellular locations. Thus, the determination of subcellular proteomes allows th...
ORGANISM(S): Bothrops jararaca (Jararaca) (Bothrops jajaraca) 
2023-09-30 | PXD040239 | Pride
Bothrops jararaca Snake venom Snake venom gland Proteome Subcellular fractionation 
ProteomicsSubcellular fractionation of Escherichia coli by differential solubilization and analysis by Maxquant LFQ-analysis
The Planctomycetes have unique cell architectures with heavily invaginated membrane networks, as here confirmed by three-dimensional models reconstructed from FIB-SEM images of Tuwongella immobilis and Gemmata obscuriglobus. We studied the subcellular proteomes of T. immobilis, and for comparison Es...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2021-04-06 | PXD022526 | Pride
Escherichia coli Subcellular fractionation 
ProteomicsSubcellular fractionation of Tuwongella immobilis by differential solubilization and analysis by Maxquant LFQ-analysis
The Planctomycetes have unique cell architectures with heavily invaginated membrane networks, as here confirmed by three-dimensional models reconstructed from FIB-SEM images of Tuwongella immobilis and Gemmata obscuriglobus. We studied the subcellular proteomes of T. immobilis, and for comparison Es...
ORGANISM(S): Tuwongella immobilis 
2021-04-06 | PXD022559 | Pride
Tuwongella immobilis Subcellular fractionation 
ProteomicsiTRAQ-based Subcellular Proteome of Avibirnavirus-infected DF-1 Cells
Infectious bursal disease virus (IBDV) enters the host cells via endocytic pathway to achieve viral replication in the cytoplasm. We performed liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling of differential abundant ...
ORGANISM(S): Gallus gallus (Chicken) 
2020-05-26 | PXD001869 | Pride
Subcellular proteomics Ibdv-infected cells Itraq Gallus gallus Lc-ms/ms 
ProteomicsMass Spectrometry-compatible Subcellular Fractionation
We developed a mass spectrometry-compatible subcellular fractionation protocol. This method involves separating cells into cytoplasmic, organellar, and nuclear fractions without ultracentrifugation.
ORGANISM(S): Homo Sapiens (human) 
2019-11-15 | PXD013581 | JPOST Repository
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