Sort   by:  
 Page size 
ProteomicsEnrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary (CHO) cells have been widely employed for production of recombinant proteins (RP) for research and therapeutic purposes. Indeed, this expression system was used for production of most of approved antibodies (84%) in 2015-2018 period. The success of this cell line for RP product...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-08-27 | PXD019778 | Pride
Organelles Subcellular fractionation Subcellular proteomics Chinese hamster ovary cells 
ProteomicsMass Spectrometry-compatible Subcellular Fractionation
We developed a mass spectrometry-compatible subcellular fractionation protocol. This method involves separating cells into cytoplasmic, organellar, and nuclear fractions without ultracentrifugation.
ORGANISM(S): Homo Sapiens (human) 
2019-11-15 | PXD013581 | JPOST Repository
ProteomicsSubcellular fractionation of Escherichia coli by differential solubilization and analysis by Maxquant LFQ-analysis
The Planctomycetes have unique cell architectures with heavily invaginated membrane networks, as here confirmed by three-dimensional models reconstructed from FIB-SEM images of Tuwongella immobilis and Gemmata obscuriglobus. We studied the subcellular proteomes of T. immobilis, and for comparison Es...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2021-04-06 | PXD022526 | Pride
Escherichia coli Subcellular fractionation 
ProteomicsSubcellular fractionation of Tuwongella immobilis by differential solubilization and analysis by Maxquant LFQ-analysis
The Planctomycetes have unique cell architectures with heavily invaginated membrane networks, as here confirmed by three-dimensional models reconstructed from FIB-SEM images of Tuwongella immobilis and Gemmata obscuriglobus. We studied the subcellular proteomes of T. immobilis, and for comparison Es...
ORGANISM(S): Tuwongella immobilis 
2021-04-06 | PXD022559 | Pride
Tuwongella immobilis Subcellular fractionation 
GenomicsSubcellular RNA localization during Drosophila embryonic neurogenesis [fractionation-Seq]
Subcellular RNA localization during Drosophila embryonic neurogenesis [fractionation-Seq]
PRJNA415601 | ENA
ProteomicsProteomic Characterization of Post-Mortem Human Brain Tissue Following Ultracentrifugation-based Subcellular Fractionation
Proteomic characterization of human brain tissue is increasingly utilized to identify potential novel biomarker and drug targets for a variety of neurological diseases. In whole tissue studies, results may be driven by changes in the proportion of the largest and most abundant organelles or tissue c...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD027456 | Pride
Subcellular fractionation Human brain Label free quantitation Alzheimer's disease 
TranscriptomicsSmall RNAs subcellular distribution in breast cancer and normal cell lines
We detect the small RNAs subcellular distribution in breast cancer cell lines MCF-7 and MDA-MB-231, and normal cell line MCF-10A. Each cell line, we detected the nuclear and cytoplasmic small RNAs expression intensity; and then we could get the nuclear-cytoplasmic-ratio.
ORGANISM(S): Homo sapiens 
2010-05-18 | E-GEOD-15937 | biostudies-arrayexpress
ProteomicsA snapshot of Bothrops jararaca snake venom gland subcellular proteome
The synthesis of snake venom proteins is subjected to finely regulated processes in the specialized secretory epithelium within the venom gland. Such processes occur within a defined time frame in the cell and at specific cellular locations. Thus, the determination of subcellular proteomes allows th...
ORGANISM(S): Bothrops jararaca (Jararaca) (Bothrops jajaraca) 
2023-09-30 | PXD040239 | Pride
Bothrops jararaca Snake venom Snake venom gland Proteome Subcellular fractionation 
ProteomicsGlobal Profiling of Protein Lactylation Across Subcellular Fractionation Methods in HCT-116
Protein lactylation is a novel post-translational modification (PTM) involved in many important physiological processes such as macrophage polarization, immune regulation and tumour cell growth. However, most studies to date have focused on the function of lactylation on histones, and little is know...
ORGANISM(S): Homo sapiens (Human) 
2024-12-15 | PXD053474 | Pride
Post-translation modification Subcellular fractionation method Lactylation 
TranscriptomicsSubcellular RNA localization during Drosophila embryonic neurogenesis [fractionation-Seq]
We isolated whole embryo, nuclear, and cytoplasmic lysates from Drosophila embryos during early and late embryogenesis In Drosophila melanogaster, cell-type specification during early nervous system development requires precise regulation of gene expression in time and space. To investigate the gene...
ORGANISM(S): Drosophila melanogaster 
2019-02-19 | GSE106094 | GEO
Sort   by:  
 Page size