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ProteomicsQuantifying missing (phospho)proteome regions with the broad-specificity protease subtilisin
Despite huge efforts to map the human proteome using mass spectrometry the overall sequence coverage achieved to date is still below 50%. Reasons for missing areas of the proteome comprise protease-resistant domains including the lack/excess of enzymatic cleavage sites, non-unique peptide sequences,...
ORGANISM(S): Homo sapiens (Human) 
2017-11-30 | PXD008068 | Pride
Novel phosphorylation sites Proteome coverage Pro-rich regions Alternative enzymes Trypsin Sample preparation Missing proteins 
ProteomicsMS characterization of spidroin-1
For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 1...
ORGANISM(S): Nephila clavipes (Golden silk orbweaver) 
2022-02-22 | PXD000057 | Pride
Major ampullate silk Nephila sp. Post-translational modifications Phosphorylation Nanolc-esi-cid/etd-ms/ms Spidroin-1 
UnknownMicroneme protein 5 regulates the activity of Toxoplasma subtilisin 1 by mimicking a subtilisin prodomain.
Not available
S-EPMC3476271 | biostudies-literature
TranscriptomicsProprotein Convertase Subtilisin/Kexin Type 7 (PCSK7) Is Essential for the Zebrafish Development and Bioavailability of Transforming Growth Factor β1a (TGFβ1a)
Proprotein convertase subtilisin/kexin (PCSK) enzymes convert proproteins into bioactive end products. Although other PCSK enzymes are known to be essential for biological processes ranging from cholesterol metabolism to host defense, the in vivo importance of the evolutionarily ancient PCSK7 has re...
ORGANISM(S): Danio rerio 
2014-01-02 | E-GEOD-42070 | biostudies-arrayexpress
UnknownOptimization of subtilisin production from Bacillus subtilis strain ZK3 and biological and molecular characterization of synthesized subtilisin capped nanoparticles.
Not available
S-EPMC10514557 | biostudies-literature
UnknownSafety assessment of subtilisin QK in rats.
Not available
S-EPMC8235616 | biostudies-literature
UnknownNew insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina.
Not available
S-EPMC2848655 | biostudies-literature
UnknownIntegrated innate mechanisms involved in airway allergic inflammation to the serine protease subtilisin.
Not available
S-EPMC4417417 | biostudies-literature
UnknownSmall RNA mediated repression of subtilisin production in Bacillus licheniformis.
Not available
S-EPMC5516005 | biostudies-literature
UnknownActive subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence.
Not available
S-EPMC92893 | biostudies-literature
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