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ProteomicsThe Ragulator complex regulates NLRP3 inflammasome activation through interaction with HDAC6
The activation of the NLRP3 inflammasome is spatiotemporally orchestrated by various organelles, but the precise roles of lysosomes are still unclear. Here we show the vital role of the Ragulator complex, a lysosomal protein, in NLRP3 inflammasome activation. Deficiency of Lamtor1, an essential comp...
ORGANISM(S): Homo sapiens (Human) 
2022-11-29 | PXD033497 | Pride
Ragulator complex Hdac6 Nlrp3 inflammasome Α-tocopherol 
ProteomicsIdentification of immunoglobulin switch (S) region binding proteins by insertional ChIP (iChIP)
We used insertional ChIP (iChIP) based approach to identify proteins that bind to mouse immunoglobulin switch (S) region. To perform iChIP, we inserted an 8X-repeat of the LexA-binding element (LexA-BE) downstream of the Sα region in CH12F3-2A cells, a mouse B-cell line. The DNA-binding domain and d...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-15 | PXD019042 | Pride
Samhd1 Silac Recombination Csr Immunoglobulin locus Lc-ms/ms 
ProteomicsPathophysiological changes in plasma and BALF of patients with ARDS based on proteomics
Acute Respiratory Distress Syndrome (ARDS) remains a significant clinical challenge, with its pathogenesis not fully understood. Proteomic analyses of plasma and bronchoalveolar lavage fluid (BALF) in patients with ARDS have been performed to uncover diagnostic and prognostic markers, although previ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD055757 | Pride
Plasma balf ards 
ProteomicsLongitudinal serum proteome analysis in COVID-19
COVID-19 is associated with endotheliopathy and coagulopathy, potentially leading to multi-organ failure. However, the direct mechanisms by which SARS-CoV-2 infection leads to endothelial damage are unclear. Here we developed an infection-competent human vascular organoid from pluripotent stem cells...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD044386 | Pride
Sars-cov-2  endotheliopathy  complement 
ProteomicsLFQ of Spot-Trap IP-MS from MEF cells expressing Irgb6-Spot
Irgb6-deficient MEFs stably expressing Spot-tagged Irgb6 were infected with or without T. gondii ME49 for 4 h and lysed in guanidine buffer (6 M guanidine-HCl, 100 mM Tris-HCl, pH 8.0, and 2 mM DTT). The lysates were diluted 8-fold with RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM EGTA, ...
ORGANISM(S): Mus Musculus (mouse) 
2023-11-21 | PXD043421 | JPOST Repository
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