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ProteomicsPhosphoproteomics of Chlamydomonas reinhardtii in photoautotrophic nitrogen-deficient conditions
Nutrient-deprived microalgae accumulate triacylglycerol (TAG) in lipid droplets. A dual-specificity tyrosine phosphorylation-regulated kinase, TAG accumulation regulator 1 (TAR1) has been shown to be required for acetate-dependent TAG accumulation and the degradation of chlorophyll and photosynthesi...
ORGANISM(S): Cellular Organisms 
2019-01-14 | PXD012311 | JPOST Repository
ProteomicsAlgal Protein Kinase, Triacylglycerol Accumulation Regulator1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen Imbalanced Conditions
Nutrient-deprived microalgae accumulate triacylglycerol (TAG) in lipid droplets. A Dual-specificity tyrosine phosphorylation-regulated kinase, TAG-accumulation-regulator-1 (TAR1) has been shown to be required for acetate-dependent TAG accumulation and degradation of chlorophyll and photosynthesis-re...
ORGANISM(S): Chlamydomonas 
2019-07-22 | PXD012391 | JPOST Repository
ProteomicsComparative proteomics of Helicobacter pylori strains reveals geographical features rather than genomic variations
Helicobacter pylori, which is known as pathogens of various gastric diseases, have many types of genome sequence variants. That is part of the reason why pathogenesis and infection mechanisms of the H. pylori-driven gastric diseases have not been well clarified yet. Here we performed a large-scale p...
ORGANISM(S): Helicobacter Pylori F16 Helicobacter Pylori F30 Helicobacter Pylori Helicobacter Pylori Ok113 Helicobacter Pylori Ok310 Helicobacter Pylori F32 
2018-12-28 | PXD011364 | JPOST Repository
ProteomicsLarge-scale Discovery of Substrates of the Human Kinome
Kinase -networks are important for cellular signal transduction. Despite tremendous efforts to uncover these signaling pathways, huge numbers of uncharacterized phosphosites still remain in the human proteome. Because of the transient nature of kinase-substrate interactions in vivo, it is almost imp...
ORGANISM(S): Homo Sapiens (human) 
2019-07-22 | PXD011366 | JPOST Repository
ProteomicsIdentifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(forskolin)
Protein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput m...
ORGANISM(S): Homo Sapiens (human) 
2018-02-14 | PXD005922 | JPOST Repository
ProteomicsIdentification of Endogenous Kinase Substrates by Proximity Labeling Combined with Kinase Perturbation and Phosphorylation Motifs
Mass spectrometry-based phosphoproteomics can identify more than 10,000 phosphorylated sites in a single experiment. But, despite the fact that enormous phosphosite information has been accumulated in public repositories, protein kinase-substrate relationships remain largely unknown. Here, we descri...
ORGANISM(S): Homo Sapiens (human) 
2021-07-16 | PXD019664 | JPOST Repository
ProteomicsMotif-centric phosphoproteomics to target kinase-mediated signaling pathways
Identifying cellular phosphorylation pathways based on kinase-substrate relationships is a critical step to understanding the regulation of physiological functions in cells. Mass spectrometry-based phosphoproteomics workflows have made it possible to comprehensively collect information on individual...
ORGANISM(S): Homo Sapiens (human) 
2021-12-01 | PXD026996 | JPOST Repository
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsMass Spectrometry-compatible Subcellular Fractionation
We developed a mass spectrometry-compatible subcellular fractionation protocol. This method involves separating cells into cytoplasmic, organellar, and nuclear fractions without ultracentrifugation.
ORGANISM(S): Homo Sapiens (human) 
2019-11-15 | PXD013581 | JPOST Repository
ProteomicsIdentifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89)
Protein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput m...
ORGANISM(S): Homo Sapiens (human) 
2018-02-15 | PXD005925 | JPOST Repository
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