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TranscriptomicsTranscriptomics analysis of gene expression in normal and METTL3 or WTAP deficient Human 293T cells
RNA was isolated from and METTL3M-oM-
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50582 | biostudies-arrayexpress
TranscriptomicsBinding motif analysis of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells
label the cells overexpressed Myc tagged METTL3 and Flag tagged WTAP with 4-SU, the RNA bound by METT3,WTAP can be got by Myc or Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library preparation using a TruSeq™ RN...
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50580 | biostudies-arrayexpress
MetabolomicsMechanisms of hepatic steatosis in chickens: integrated analysis of the host genome, molecular phenomes and gut microbiome

Hepatic steatosis is the initial manifestation of abnormal liver functions and often leads to liver diseases such as non-alcoholic fatty liver disease in humans and fatty liver syndrome in animals. In this study, we conducted a comprehensive analysis of a large chicken population consisting of 70...

2023-07-24 | MTBLS7808 | MetaboLights
TranscriptomicsIntegration of developmental and nutritional cues determines timing of female flower opening in cucumber
The timing of flower opening is essential for pollination and thus seed production. Despite flower opening has been a long lasting topic for plant biologists, the underling molecular mechansim remains elusive. Here we used a unique cucumber line ‘6457’ that spontaneously produces super ovary wit...
ORGANISM(S): Cucumis sativus 
2016-07-25 | E-GEOD-76358 | biostudies-arrayexpress
UnknownGenetic Factors Underlying Single Fiber Quality in A-Genome Donor Asian Cotton (Gossypium arboreum).
Not available
S-EPMC8689003 | biostudies-literature
ProteomicsCombine in silico target fishing, Comparative proteomics analysis and in vitro assays to identify antibacterial effects of a TCM (the leaves of Strobilanthes cusia Kuntze) against the Penicillin resistant Streptococcus pneumoniae
The anti-bacterial mechanism of the leaves of Strobilanthes cusia Kuntze against the Penicillin-resistant Streptococcus pneumoniae were analyzed by the comparative proteomics
ORGANISM(S): Streptococcus Pneumoniae 23f 
2022-12-18 | PXD038886 |
ProteomicsRBIS regulates ribosome biogenesis to affect progression in lung adenocarcinoma
Transient overexpress RBIS and control plasmid in H293T cells and collect cells after 48 hours, then performed IP assays using anti-Flag beads subjected samples to LC-MSMS to screen proteins interacting with RBIS.
ORGANISM(S): Homo Sapiens 
2024-10-23 | PXD057090 |
ProteomicsThe deubiquitinase USP13 maintains cancer cell stemness by promoting FASN stability in small cell lung cancer
USP13 is significantly amplified in over 20% of lung cancer patients and critical for tumor progression. However, the functional role of USP13 in small cell lung cancer (SCLC) remains largely unclear. In this study, we found that the deubiquitinase USP13 is highly expressed in SCLC tumor samples and...
ORGANISM(S): Homo Sapiens 
2022-04-06 | PXD032993 |
ProteomicsPulmonary and Renal Long COVID at Two-year Revisit
To outline the temporal changes of long COVID, we profiled serum proteomes (dataset SP2) and urine proteomes (dataset UP2). The SP2 were measured for 37 patients during the disease (T1), the convalescence (T2), and at the 1-year revisit (T3); the UP2 were measured for 28 patients during the disease ...
ORGANISM(S): Homo Sapiens 
2024-06-04 | PXD027557 |
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