To identify the unknown receptor, we extracted the membranous proteins from HCT8 cells treated with rCyr61.Then the proteins were subjected to mass spectrometry
To identify the unknown interacting proteins with GPT1, we extracted the proteins from HCT8 cells and performed co-immunoprecipitation assays with GPT1 antibody. Then the proteins were subjected to mass spectrometry.
To identify the unknown interacting proteins with circPLCE1-411, we extracted the proteins from HCT8 cells and performed co-immunoprecipitation assays with Flag antibody. Then the proteins were subjected to mass spectrometry
To identify the unknown interacting proteins with circINSIG1-121, we extracted the proteins from HCT8 cells and performed co-immunoprecipitation assays with circINSIG1-121 antibody. Then the proteins were subjected to mass spectrometry
To identify the potential circGlis3 binding proteins, we extracted the total protein from MS-1 cells and performed RNA Pull-down assay. Then RNA-interacting proteins were subjected to mass spectrometry.