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TranscriptomicsHigh-throughput sequencing of small RNAs in Medicago truncatula
Medicago truncatula endogenous small RNAs The dataset contains Medicago truncatula Gaertn. cv. Jemalong endogenous small RNA sequences in the range 18-28 nucleotides. High-throughput Solexa/Illumina sequencing was carried out at the Sainsbury Laboratory, Norwich, UK. Please see www.illumina.com for...
ORGANISM(S): Medicago truncatula 
2009-11-09 | E-GEOD-13761 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of small RNAs in Vitis vinifera
Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high thr...
ORGANISM(S): Vitis vinifera 
2010-04-29 | E-GEOD-18405 | biostudies-arrayexpress
TranscriptomicsmicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera
Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel ...
ORGANISM(S): Vitis vinifera 
2010-04-29 | E-GEOD-18406 | biostudies-arrayexpress
TranscriptomicsHigh-throughput pyrosequencing of endogenous small RNAs from Solanum lycopersicum leaf and fruit
Comparison of the endogenous small RNA content of tomato leaves and fruits. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to 454 high throughput pyrosequencing. Please see www.454...
ORGANISM(S): Solanum lycopersicum 
2008-08-21 | E-GEOD-12081 | biostudies-arrayexpress
TranscriptomicsStructural and Functional Analysis of Viral siRNAs using 454 sequencing
We profiled vsiRNAs using two different high-throughput sequencing platforms and also developed a hybridisation-based array approach. The profiles obtained through the Solexa platform and by hybridisation were very similar to each other but different from the 454 profile. Both deep sequencing techni...
ORGANISM(S): Nicotiana benthamiana 
2010-04-11 | E-GEOD-17164 | biostudies-arrayexpress
TranscriptomicsmicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum
Solanum lycopersicum RNA degradome sequencing Isolated polyadenylated RNA from total RNA extracts of Solanum lycopersicum, were ligated to 5'-adapter that includes an MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were pur...
ORGANISM(S): Solanum lycopersicum 
2012-08-29 | E-GEOD-22236 | biostudies-arrayexpress
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