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GenomicsCritical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency (Chip-Seq)
We recently showed that some human induced pluripotent stem cell (iPSC) clones were defective in neural differentiation and were marked with the activation of long term repeats (LTRs) of human endogenous retroviruses (HERVs). We herein demonstrated that these LTRs were transiently overexpressed duri...
ORGANISM(S): Homo sapiens 
2014-08-04 | E-GEOD-56567 | biostudies-arrayexpress
TranscriptomicsMaturation,not initiation, is major roadblock during reprogramming toward pluripotency from human fibrobalsts
Reprogrammed cells are reverted to non reprogrammed cell fate in a maturation step of reprogramming. Cell fate changes during reprogramming from fibroblasts to iPSC , N=60 fibrobalst lines, N=13 Undifferentiated pluripotent stem cells, N=16 Nascent reprogrammed cells, N=31
ORGANISM(S): Homo sapiens 
2013-07-01 | E-GEOD-47489 | biostudies-arrayexpress
TranscriptomicsCritical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency (RNA-Seq)
We recently showed that some human induced pluripotent stem cell (iPSC) clones were defective in neural differentiation and were marked with the activation of long term repeats (LTRs) of human endogenous retroviruses (HERVs). We herein demonstrated that these LTRs were transiently overexpressed duri...
ORGANISM(S): Homo sapiens 
2014-08-04 | E-GEOD-56568 | biostudies-arrayexpress
TranscriptomicsInduction of pluripotency in human somatic cells via a primitive streak-like state
During mammalian embryonic development, the primitive streak is initiates the differentiation of pluripotent epiblast cells into germ layers. Pluripotency can be reacquired in committed somatic cells using a combination of handful transcription factors, such as OCT3/4, SOX2, KLF4 and c-MYC (hereafte...
ORGANISM(S): Homo sapiens 
2014-04-24 | E-GEOD-50206 | biostudies-arrayexpress
ProteomicsA stress-reduced passaging technique improves the viability of human pluripotent cells
Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more effic...
ORGANISM(S): Homo Sapiens (human) 
2022-01-23 | PXD028731 | JPOST Repository
ProteomicsPost-transcriptionally regulated genes are essential for pluripotent stem cell survival
Here, by combining transcriptome and proteome profiling, we identified 228 post-transcriptionally regulated genes with strict regulation at the protein level in PSCs. Among them, 84 genes were found necessary for the survival of several cell types including PSCs, and 20 genes were identified as spec...
ORGANISM(S): Homo Sapiens (human) 
2022-05-10 | PXD028489 | JPOST Repository
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