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Loss of pancreatic Beta cell mass, identity, and function contribute to the development of diabetes. Here, we show that the endoplasmic reticulum (ER) calcium sensor, stromal interaction molecule 1 (STIM1), is critical for the maintenance of Beta cell function in female mice. When mice with Beta cel...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2022-07-20 | MSV000089927 | MassIVE
(1) Secreted materials from the venom gland: 50 venom glands were cultured in 20 μL PBS for 2 hours. The cultured media were collected. Three biological replications were analyzed. (2) Lysates of the venom glands: 50 venom glands were squashed in 50 μL PBS and centrifuged. The supernatants were coll...
ORGANISM(S): Cellular Organisms 
FLAG-tagged 5MP (h5MP1/BZW2 from human and 5MP/Kra from fly) was ectopically expressed in relevant host cells (HEK293T for human and S2 for fly) by transient transfection, and one-step affinity-purified by anti-FLAG affinity resin.The one-step affinity purification products were resolved by SDS-PAGE...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
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