Data interpreting of PDX proteome is still a problem because with the growth of human cancerous tissue in an immunodeficient mouse, the endothelial cells and fibroblasts from the host mouse replaced human stromal components, making PDX sample a mixture of human and mouse cells. In this study, we cre...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse)
Cholesterol of the host macrophage membrane is vital for mycobacterial infection, replication, and persistence. During chronic infection within host lung tissues, cholesterol facilitates the phagocytosis of mycobacteria into macrophages. Cholesterol degradation leads to increased flux of acetyl-coenz...
In this study, we explored BoxCar method in urine proteome sample from diabetic patients and systematically evaluated the advantages of BoxCar method in proteome analysis in diabetes urine compared to standard data method.
Open (mass tolerant) search of tandem mass spectra shows great potential in the comprehensive detection of post-translational modifications in shotgun proteomics. However, this search strategy has not been widely used by the community, and one bottleneck of it is the lack of appropriate algorithms f...
So far, high-efficiency and high-throughput analysis of C-terminal peptides is still full of challenges. In order to make the chemical derivatization-based strategies more practical and efficient, in this study we developed a new method, named 2-pyridinecarboxaldehyde (2PCA) and biotin labeling bas...
we developed a new method for C-terminomics, in which LysargiNase and chemical derivatization were integrated to increase the identification confidence.
In this study, we used a novel strategy of pSILAC method coupled with two complementary digestion approaches to find potential substrates of E7070, and reveals PRPF39 as E7070-dependent DCAF15 substrate.
AMPK is a conservative energy sensor that plays roles in diverse cellular processes via direct phosphorylation on various substrates. Emerging studies have demonstrated the regulatory roles of AMPK in DNA damages, but the underlying mechesiams remains to be fully understood. Our studies provided a s...
