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ProteomicsExtending the Separation Space with Trapped Ion Mobility Spectrometry Improves the Accuracy of Isobaric Tag-based Quantitation in Proteomic LC/MS/MS
Two-dimensional separation by nano-LC and trapped ion mobility spectrometry (TIMS) prior to Q-TOF tandem mass spectrometry significantly improves the accuracy of isobaric tag-based quantitation in proteome analysis without the need for additional measurement time for TIMS insertion. The obtained pea...
ORGANISM(S): Cellular Organisms 
2020-05-22 | PXD018650 | JPOST Repository
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-27 | MSV000082855 | MassIVE
UnknownImproved isobaric tandem mass tag quantification by ion mobility mass spectrometry.
Not available
S-EPMC4000571 | biostudies-literature
ProteomicsExpanding the Landscape of Aging via Orbitrap Astral Mass Spectrometry and Tandem Mass Tag (TMT) Integration
Aging results in progressive decline in physiological function due to the deterioration of essential biological processes, such as transcription and RNA splicing, ultimately increasing mortality risk. Although proteomics is emerging as a crucial tool for elucidating the molecular underpinnings of ag...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-03 | PXD058160 | Pride
Tandem mass tag(tmt) Proteomics Agining Mouse Orbitrap astral mass spectrometer 
UnknownDeep Profiling of Microgram-Scale Proteome by Tandem Mass Tag Mass Spectrometry.
Not available
S-EPMC8262114 | biostudies-literature
UnknownAssessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling.
Not available
S-EPMC7357674 | biostudies-literature
UnknownAutomated Sample Preparation Workflow for Tandem Mass Tag-Based Proteomics.
Not available
S-EPMC10557128 | biostudies-literature
ProteomicsAutomated sample preparation workflow for tandem mass tag-based proteomics
Although tandem mass tag (TMT)-based isobaric labeling has become a powerful technique for multiplexed protein quantitation, it has not been easy to automate the workflow for widespread adoption. This is because preparation of TMT labeled peptide samples involves multiple steps ranging from protein ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD040788 | Pride
Automation Tandem mass tags Proteomics 
Unknown29-Plex tandem mass tag mass spectrometry enabling accurate quantification by interference correction.
Not available
S-EPMC9588555 | biostudies-literature
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