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ProteomicsMultiplexed complexome profiling using tandem mass tags
Complexome profiling is a rapidly spreading, powerful approach to gain insight into the nature of protein complexes. It identifies and quantifies protein complexes separated into multiple fractions of increasing molecular mass using mass spectrometry-based, label-free bottom-up proteomics. Complexom...
ORGANISM(S): Homo sapiens (Human) 
2021-08-20 | PXD023211 | Pride
Complexome profiling  multiplexed complexomics  tandem mass tags  protein complexes 
ProteomicsAnalysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT)
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-27 | MSV000082855 | MassIVE
UnknownTime-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing.
Not available
S-EPMC5141271 | biostudies-literature
ProteomicsChicken Tandem-Mass-Tag quantitative mass spectrometry analysis
About “MS210144-1 to MS210144-12”: 6 samples: cell body-1#, cell body-2#, cell body-3#, Migrasome-1#, Migrasome-2#, Migrasome-3#, were labeled with tandem mass tags (TMT) reagents and then mixed. These combined TMT-labeled peptides were separated into 12 fractions (shown in list: from MS210144-1 to ...
ORGANISM(S): Gallus gallus (Chicken) 
2022-10-12 | PXD036647 | Pride
Tandem mass tags 
ProteomicsSerum proteomic analysis by tandem mass tags-based quantitative proteomics in pediatric obstructive sleep apnea
Pediatric obstructive sleep apnea (OSA) is a frequent respiratory disorder with an estimated prevalence of 3–6% in the general population. However, the underlying pathophysiology of OSA remains unclear. Recently, proteomic analysis using high-resolution and high-throughput mass spectrometry has been...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD032734 | Pride
Obstructive sleep apnea  proteomic analysis  tandem mass tags  differentially expressed proteins  serum  experimental validation 
ProteomicsMultiplexed quantitation of post-translational modified peptides in single cells using triggered MS/MS combined with super heavy tandem mass tags
Recent advances in mass spectrometry now allow unbiased proteome profiling of thousands of proteins from single cells using both label-free and labeling approaches. However, a major limitation of unbiased approaches is missing data, which worsens as the sample size increases. In addition, the reprod...
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD073405 | Pride
Ptm Surequant Single cell proteomics Super heavy tandem mass tags 
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo sapiens (Human) 
2016-07-01 | PXD003346 | Pride
Hcd Tandem mass tags Quantitative mass spectrometry Label-free quantification 
ProteomicsMass spectrometry-based Quantitative RNA Modification Mapping Using Isobaric Tandem Mass Tags
Here, we present an RNA-specific isobaric tandem mass tagging (RMT) approach that enables the quantitative mapping of RNA modifications at an omics scale with high chemical accuracy. These RNA mass tags, derived and optimized from proteomics isobaric tags, are incorporated into an end-to-end workflo...
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) 
2026-07-01 | PXD066919 | Pride
Rna modification Tmt Tandem mass tags Itraq 
ProteomicsReproducible Workflow for Multiplexed Deep-Scale Proteome and Phosphoproteome Analysis of Tumor Tissues by Liquid Chromatography-Mass Spectrometry

Mertins P, Tang LC, Krug K, Clark DJ, Gritsenko MA, Chen L, Clauser KR, Clauss TR, Shah P, Gillette MA, et al., Nat Protoc. 2018 Jul 9. doi: 10.1038/s41596-018-0006-9

Here we present an optimized workflow for gl...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2019-08-30 | MSV000084265 | MassIVE
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