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Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsTandem mass tag labeling to characterize muscle-specific proteome change in beef during early postmortem
Bovine longissimus lumborum (LL) and psoas major (PM) muscles biopsy samples were collected from four carcasses (n = 4) at 45 min, 12 h, and 36 h postmortem from a commercial beef processing facility. Proteome profile variation between beef LL and PM during the early postmortem period were evaluated...
ORGANISM(S): Bos taurus (Bovine) 
2020-02-19 | PXD017535 | Pride
Tmt Muscle-to-meat conversion 
UnknownA benchmarking protocol for intact protein-level Tandem Mass Tag (TMT) labeling for quantitative top-down proteomics.
Not available
S-EPMC9587358 | biostudies-literature
ProteomicsOptimization of Higher-Energy Collisional Dissociation Fragmentation Energies for Intact Protein-level Tandem Mass Tag Labeling
Isobaric chemical tag labeling (e.g., iTRAQ and TMT) is a commonly used approach in quantitative proteomics research. Typically, peptides are covalently labeled with isobaric chemical tags, and quantification is enabled through detection of low-mass reporter ions generated after MS2 fragmentation. R...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD037491 | Pride
Protein-level tmt labeling  top-down proteomics  quantitative proteomics  hcd 
UnknownTandem Mass Tag Labeling Facilitates Reversed-Phase Liquid Chromatography-Mass Spectrometry Analysis of Hydrophilic Phosphopeptides.
Not available
S-EPMC7197904 | biostudies-literature
ProteomicsAutomated sample preparation workflow for tandem mass tag-based proteomics
Although tandem mass tag (TMT)-based isobaric labeling has become a powerful technique for multiplexed protein quantitation, it has not been easy to automate the workflow for widespread adoption. This is because preparation of TMT labeled peptide samples involves multiple steps ranging from protein ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD040788 | Pride
Automation Tandem mass tags Proteomics 
UnknownQuantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling.
Not available
S-EPMC8323476 | biostudies-literature
UnknownResin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation.
Not available
S-EPMC8828046 | biostudies-literature
ProteomicsmTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Silac Tmt Isobaric Sh-sy5y Svgp12 Metabolic 
ProteomicsExpanding the Landscape of Aging via Orbitrap Astral Mass Spectrometry and Tandem Mass Tag (TMT) Integration
Aging results in progressive decline in physiological function due to the deterioration of essential biological processes, such as transcription and RNA splicing, ultimately increasing mortality risk. Although proteomics is emerging as a crucial tool for elucidating the molecular underpinnings of ag...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-03 | PXD058160 | Pride
Tandem mass tag(tmt) Proteomics Agining Mouse Orbitrap astral mass spectrometer 
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