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GenomicsDrosophila melanogaster strain:w
tejas and tapas function for female germline maintenance and piRNA production in D. melanogaster
PRJNA255443 | ENA
ProteomicsTAP-GluN1, PSD95-TAP, WT (control) BNP-LC-MS/MS proteomics
TAP-GluN1 (840 kDa and 1.5 MDa), PSD95-TAP (1.5 MDa) and WT (control; 1.5 MDa) native complexes from Grin1^TAP/TAP, Dlg4^TAP/TAP, and WT mouse forebrain (cortex and hippocampus), respectively. Purified samples were seperated by blue native PAGE. Bands were excised, digested with trypsin. Peptides we...
ORGANISM(S): Mus musculus (Mouse) 
2016-04-20 | PXD000011 | Pride
Native complexes Synapse Nmdar 
TranscriptomicsMicroarray analysis of TAP::ALG-1 associated RNAs isolated from synchronized 'wild-type' animals and 'mir-58' mutants
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein (C. elegans strain WS4303). We crossed the TAP::ALG-1 transgene into the mir-58(n4640) mutant background to generate the strain WS5041...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32942 | biostudies-arrayexpress
TranscriptomicsPatterns of dioxin-altered mRNA expression in livers of dioxin-sensitive versus dioxin-resistant rats
The dioxin congener 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes a wide range of toxic effects in rodent species, all of which are mediated by a ligand-dependent transcription-factor, the aryl hydrocarbon receptor (AHR). The Han/Wistar (Kuopio) (H/W) strain shows exceptional resistance to many ...
ORGANISM(S): Rattus norvegicus 
2011-11-14 | E-GEOD-13513 | biostudies-arrayexpress
TranscriptomicsRNAs associated with TAP-ZC3H28 in Trypanosoma brucei
RNAs associated with TAP-ZC3H28
ORGANISM(S): Trypanosoma brucei brucei 
2021-07-31 | E-MTAB-10674 | biostudies-arrayexpress
TranscriptomicsGene expression profile in breast cancer cell lines using miRNA over-expression
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag at the C-term (C-TAP-ER-beta). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) for ...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-MTAB-1493 | biostudies-arrayexpress
TranscriptomicsAnalysis to evaluate the presence of 5’ cap in intragenic lncRNAs
RNA aliquots were digested with 5’-phosphate-dependent exonuclease (5'-exo) following pre-treatment with tobacco acid pyrophosphatase (TAP) to release the 5’ cap and render the RNA susceptible to 5’ exonuclease digestion (TAP+/5'-exo+). Two color array hybridizations were performed and TAP+/5'-exo+...
ORGANISM(S): Homo sapiens 
2015-06-30 | E-GEOD-62984 | biostudies-arrayexpress
TranscriptomicsGene expression profile in a breast cancer cell line upon hormonal treatments
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-alpha tagged with TAP-tag at the C-term (C-TAP-ER-alpha). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) fo...
ORGANISM(S): Homo sapiens 
2012-06-25 | E-MTAB-1196 | biostudies-arrayexpress
GenomicsLong range spreading of dosage compensation in Drosophila captures transcribed autosomal genes inserted on X
Dosage compensation in D. melanogaster males is achieved via targeting of the MSL complex to X chromosomal genes. This is proposed to involve initial sequence-specific recognition of the X at ~150-300 chromatin entry sites, and subsequent spreading to nearby active genes. Here we test a model in whi...
ORGANISM(S): Drosophila melanogaster 
2009-08-20 | E-GEOD-17729 | biostudies-arrayexpress
GenomicsGenomic targets sites of the Candida glabrata transcription factor Pdr1
Pdr1 is the major regulator of azole resistance in the fungal pathogen Candida glabrata. Earlier experiments demonstrated that expression of Pdr1 itself is increased when cells lose their mitochondrial genome (rho0). Here we use chromatin immunoprecipitation coupled with highthroughput sequencing ...
ORGANISM(S): Candida glabrata CBS 138 
2014-09-12 | E-GEOD-59839 | biostudies-arrayexpress
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