Sort   by:  
 Page size 
TranscriptomicsMicroarray analysis of TAP::ALG-1 associated RNAs isolated from synchronized 'wild-type' animals and 'mir-58' mutants
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein (C. elegans strain WS4303). We crossed the TAP::ALG-1 transgene into the mir-58(n4640) mutant background to generate the strain WS5041...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32942 | biostudies-arrayexpress
TranscriptomicsPurification of TAP::ALG-1 complexes and associated RNA from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2) animals
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein We purified TAP::ALG-1 complexes from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2, serving as a mock control) animals...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | E-GEOD-32941 | biostudies-arrayexpress
ProteomicsCharacterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in ...
ORGANISM(S): Homo sapiens (Human) 
2018-12-17 | PXD011432 | Pride
Chaperone  heat shock protein 90  hek293t  kidney stone  sirna  tap tag 
ProteomicsCharacterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-12-17 | MSV000083252 | MassIVE
ProteomicsIdentification of Mitogen-Activated Protein Kinase Phosphatase-1 (MKP-1) protein partners using Tandem Affinity Purification and Mass Spectrometry
According to the World Health Organization Report, depressive disorders affect about 10% of the population. The molecular mechanism of the pathogenesis of depression is still not well understood. The new findings point to phosphatases as potential targets for effective depression therapy. The aim of...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2023-01-19 | MSV000091093 | MassIVE
OtherPurification of TAP::ALG-1 complexes and associated RNA from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2) animals
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein We purified TAP::ALG-1 complexes from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2, serving as a mock control) animals...
ORGANISM(S): Caenorhabditis elegans 
2012-03-01 | GSE32941 | GEO
TranscriptomicsA toolbox for epitope-tagging and genome-wide location analysis in Candida albicans
Background : Candida albicans is a diploid pathogenic fungus not yet amenable to routine genetic investigations. Understanding aspects of the regulation of its biological functions and the assembly of its protein complexes would lead to further insight into the biology of this common disease-causing...
ORGANISM(S): Candida albicans 
2010-05-16 | E-GEOD-13427 | biostudies-arrayexpress
ProteomicsTAP-GluN1, PSD95-TAP, WT (control) BNP-LC-MS/MS proteomics
TAP-GluN1 (840 kDa and 1.5 MDa), PSD95-TAP (1.5 MDa) and WT (control; 1.5 MDa) native complexes from Grin1^TAP/TAP, Dlg4^TAP/TAP, and WT mouse forebrain (cortex and hippocampus), respectively. Purified samples were seperated by blue native PAGE. Bands were excised, digested with trypsin. Peptides we...
ORGANISM(S): Mus musculus (Mouse) 
2016-04-20 | PXD000011 | Pride
Native complexes Synapse Nmdar 
TranscriptomicsRNAs associated with TAP-ZC3H28 in Trypanosoma brucei
RNAs associated with TAP-ZC3H28
ORGANISM(S): Trypanosoma brucei brucei 
2021-07-31 | E-MTAB-10674 | biostudies-arrayexpress
ProteomicsIntegrated Tandem Affinity Protein Purification Using the Polyhistidine plus Extra 4 Amino Acids (HiP4) Tag System
Interactome analysis of HBx using a novel tag system (HiP4-tag system).
ORGANISM(S): Homo Sapiens (human) 
2023-02-28 | PXD036256 | JPOST Repository
Sort   by:  
 Page size