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TranscriptomicsTh single cell RNA-Seq
Th2 cells regulate helminth infections, allergic disorders, tumor immunity and pregnancy by secreting various cytokines. It is likely that there are undiscovered Th2-signaling molecules. While steroids are known to be immunoregulators, de novo steroid production from immune cells has not been previo...
ORGANISM(S): Mus musculus 
2014-04-25 | E-MTAB-2512 | biostudies-arrayexpress
TranscriptomicsA spatial multi-omics atlas of the human lung reveals a novel immune cell survival niche - Visium
Multiple distinct cell types of the human lung and airways have been defined by single cell RNA sequencing (scRNAseq). Here we present a multi-omics spatial lung atlas to define novel cell types which we map back into the macro- and micro-anatomical tissue context to define functional tissue microen...
ORGANISM(S): Homo sapiens 
2022-01-06 | E-MTAB-11640 | biostudies-arrayexpress
TranscriptomicsSingle-cell and single-nucleus RNAseq of the human and mouse skeletal muscle across age
This study aims to characterize the diversity of cell types in human skeletal muscle across age using two complementary technologies: single-cell and single-nucleus sequencing, which provide a comprehensive coverage of cell types in the muscle. We leveraged the aforementioned datasets to study chang...
ORGANISM(S): Mus musculus 
2024-03-07 | E-MTAB-13874 | biostudies-arrayexpress
TranscriptomicsSingle-cell sequencing of developing human gut reveals transcriptional links to childhood Crohn’s disease
In this study, we performed single-cell transcriptional profiling of human embryonic and fetal gut samples obtained from 9 human embryos spanning ages 6-10 PCW and three regions (duo-jejunum, ileum and colon). Additionally, we profile mucosal biopsies from the terminal ileum of healthy children aged...
ORGANISM(S): Homo sapiens 
2020-12-22 | E-MTAB-8901 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptional landscape of human embryonic limb development - Visium
We performed spatial transcriptomic experiments using the 10x Visium assay, generating high-quality transcriptomic profiles for samples from PCW5 to PCW8
ORGANISM(S): Homo sapiens 
2022-04-15 | E-MTAB-10367 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptional landscape of human embryonic limb development
Human limbs emerge during the fourth post-conception week as mesenchymal buds, which develop into fully formed limbs over the subsequent months. This process is orchestrated by numerous temporally and spatially restricted gene expression programmes, making congenital alterations in phenotype common....
ORGANISM(S): Homo sapiens 
2022-04-15 | E-MTAB-8813 | biostudies-arrayexpress
TranscriptomicsAcquisition of alveolar fate and differentiation competence by human fetal lung epithelial progenitor cells
Our data show that Wnt and FGF signalling, and the downstream transcription factors NKX2.1 and TFAP2C, promote human alveolar or airway fate respectively. Moreover, we have functionally validated cell-cell interactions in human lung alveolar patterning. We show that Wnt signalling from differentiati...
ORGANISM(S): Homo sapiens 
2022-02-28 | E-MTAB-11435 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptional landscape of human embryonic limb development - Mouse
To investigate into the evolutionary conversation of the single-cell transcriptome of human fetal limbs, especially the principles of forelimb and hindlimb specification and proximal-distal axis establishment, we collected the forelimbs and hindlimbs of mouse embryos matching human samples. We disse...
ORGANISM(S): Mus musculus 
2022-04-15 | E-MTAB-10514 | biostudies-arrayexpress
TranscriptomicsA high-resolution single-cell multiomic atlas of the human fetal lung - Visium
Fetal lung samples at 12–20 post conception week (pcw) from the HDBR, up to 0.5cm3 in size, were embedded in OCT and flash-frozen in dry-ice cooled isopentane. Twelve-micron cryosections were cut onto Visium slides, haematoxylin and eosin stained and imaged at 20X magnification on a Hamamatsu Nanozo...
ORGANISM(S): Homo sapiens 
2022-01-27 | E-MTAB-11265 | biostudies-arrayexpress
TranscriptomicsA high-resolution single-cell multiomic atlas of the human fetal lung - Organoid
Individual TFs were overexpressed in fetal lung tip organoids from a doxycycline-inducible construct for 3 days, and organoids were maintained in the self-renewing (tip cell-promoting) medium throughout to rigorously assay the lineage-determining competence of the TF, followed by scRNA-seq. ASCL1, ...
ORGANISM(S): Homo sapiens 
2022-01-27 | E-MTAB-11267 | biostudies-arrayexpress
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