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ProteomicsMass spectrometry-driven discovery of neuropeptides mediating nictation behavior of nematodes
Very little is known about the genetic modulation of nictation, a host-finding strategy used by infective juveniles of many pathogenic nematodes, as well as a phoretic strategy of Caenorhabditis elegans dauers. We here use a mass spectrometry-driven approach to find neuropeptides involved in the mod...
ORGANISM(S): Steinernema carpocapsae 
2022-12-08 | PXD034629 | Pride
Nictation Data-dependent acquisition Steinernema carpocapsae Peptidomics Parallel reaction monitoring Caenorhabditis elegans 
ProteomicsMass spectrometric evidence for neuropeptide-amidating enzymes in C. elegans
Neuropeptides constitute a vast and functionally diverse family of neurochemical signaling molecules, and are widely involved in the regulation of various physiological processes. The nematode C. elegans is well-suited for the study of neuropeptide biochemistry and function, as neuropeptide biosynth...
ORGANISM(S): Caenorhabditis elegans 
2018-03-12 | PXD008942 | Pride
Peptidomics  mass spectrometry  neuropeptide  amidation  copper monooxygenase  phm  pal  pam  caenorhabditis elegans  lc-orbitrap ms 
ProteomicsYolk-deprived Caenorhabditis elegans secure brood size at the expense of competitive fitness
Oviparous animals support reproduction via the incorporation of yolk as a nutrient source into the eggs. In Caenorhabditis elegans, however, yolk proteins seem dispensable for fecundity, despite constituting the vast majority of the embryonic protein pool and acting as carriers for nutrient-rich lip...
ORGANISM(S): Caenorhabditis elegans 
2023-04-12 | PXD041110 | Pride
C. elegans Tmt Yolk deprivation 
ProteomicsCEH-60/PBX regulates vitellogenesis and cuticle formation through interaction with UNC-62/MEIS in Caenorhabditis elegans
The onset of sexual maturity involves dramatic changes in physiology and gene expression in many animals. A textbook example is the production of enormous amounts of yolk proteins destined for the nascent oocytes, termed vitellogenesis. We previously identified the rapidly evolving PBC-class Hox-cof...
ORGANISM(S): Caenorhabditis elegans 
2019-09-25 | PXD013584 | Pride
Caenorhabditis elegans Yolk proteins Tmt Ceh-60 Vitellogenesis Collagen Cuticle 
GenomicsMethylation profiling of C.elegans to identify CEH-60/PBX binding sites
CEH-60 binding profile by comparison of DNA methylation of a C. elegans strain expression ceh-60::dam to a control strain expressing gfp::dam. Sequencing libraries prepared using NEBNext Singleplex oligos for Illumina®. Data analysis performed with GeneDamIDseq (Sharma, Ritler, and Meister 2016).
ORGANISM(S): Caenorhabditis elegans 
2020-11-16 | E-MTAB-9539 | biostudies-arrayexpress
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