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Proteomics[HPP-2.0] Matrix Thermal Shift Assay for Fast Construction of Multidimensional Ligand–Target Space
Existing thermal shift-based mass spectrometry approaches are able to identify target proteins without chemical modification of the ligand, but they are suffering from complicated workflows with limited throughput. Herein, we present a new thermal shift-based method, termed matrix thermal shift assa...
ORGANISM(S): Homo Sapiens 
PXD045941 |
MetabolomicsIn plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids (GC- and LC-MS assays)

Tapping into the metabolic cross-talk between a host and its virus can reveal unique strategies employed during infection. Viral infection is a dynamic process that generates an evolving metabolic landscape. Gaining a continuous view into the infection process is highly challenging and is limited...

2018-11-29 | MTBLS767 | MetaboLights
ProteomicsProteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Despite the growing interest in using chemical genetics in plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution massspectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD030309 | Pride
Brassinosteroids Auxin Chemical genetics Cellular thermal shift assay 
ProteomicsIdentifying Functionally Relevant Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay
In this study, we sought to identify how protein thermal stability integrates with changes in cell signaling and protein abundance. We developed a pipeline to investigate changes in protein abundance and thermal stabilite by combining the protein abundance and thermal shift measurements into the sam...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-17 | PXD050772 | Pride
Phosphoproteomics T cell activation Thermal stability 
ProteomicsLarge-scale characterization of drug mechanism of action using proteome-wide thermal shift assays - Revisions
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD053138 | Pride
Thermal shift assay Proteomics Drug discovery 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsLarge-scale characterization of drug-induced changes in thermal stability using proteome-wide thermal shift assays
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD048009 | Pride
Mechanism of action Drug development 
TranscriptomicsEZH2 inhibitor efficacy in non-Hodgkin lymphoma does not require suppression of H3K27 mono-methylation [RNA-Seq]
Here we report the discovery of highly potent and selective EZH2 small molecule inhibitors, their validation by a cellular thermal shift assay, their application across a large lymphoma cell panel and their efficacy in GCBDLBCL xenograft models. RNA-seq of KARPAS-422 cell line RNA, in duplicate, tre...
ORGANISM(S): Homo sapiens 
2015-04-13 | E-GEOD-62056 | biostudies-arrayexpress
UnknownHomogeneous Assay for Target Engagement Utilizing Bioluminescent Thermal Shift.
Not available
S-EPMC6004564 | biostudies-literature
ProteomicsThermal proteome profiling and proteome analysis using high-definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next-generation galeterone analog VNPP433-3β in castration-resistant prostate cancer
Cholesterol (CHOL) homeostasis is significantly modulated in prostate cancer (PCa) suggesting an active role in PCa development and progression. Several studies indicate a strong correlation between elevated CHOL levels and increased PCa risk and severity. Inhibition of CHOL biosynthesis at differen...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD060338 | Pride
Next generation galeterone analog Cyp51a1 Thermal proteome profiling Cholesterol Prostate cancer Lanosterol synthase 
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