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A2ML1 (wildtype and H1084N) and A2M (wildtype and N1088H) were activated by chymotrypsin, allowing their thiol ester to react with protease, water, or the added substrates glycerol or glycine.
ORGANISM(S): Homo sapiens (Human) 
2020-09-25 | PXD020826 | Pride
In-gel digest of a cross-linked product band was used to identify an internal isopeptide cross-link formed in A2M TR K704 upon bait region cleavage by trypsin. PRM was used to quantify the thiol ester in native PAGE bands of A2M nEXT and EXT mutants.
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023651 | Pride
This proteomic study This proteomic study focuses on the role of reversible thiol oxidation after hexyl aminolevulinate mediated PDT (HAL-PDT). The human epidermoid carcinoma cell line A431 was used as model system and red light as light source, a clinical relevant in vitro model. The light dose dep...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-04-01 | MSV000080866 | MassIVE
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