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E. coli MG1655 was grown on M9 minimal medium supplemented with 15mM glucose and either 0, 10, 50 or 100 mM acetate. Transcriptomic analysis from exponential growing cells was performed using Agilent microarray A-MEXP-2232. This work was done with the help of students during practicals. Consequentl...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
LC-MS/MS of Prss37 WT and KO mice male reproductive tract. Title: Molecular dissection and testing of PRSS37 function through LC-MS/MS and the generation of a PRSS37 humanized mouse model Authors: Courtney Sutton1,2, Kaori Nozawa1,2, Katarzyna Kent1,2, Alexander Saltzman3, Mei Leng3, Sureshbabu Na...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-04-07 | MSV000091662 | MassIVE
The appended raw files, csv files and other documents were deposited into the public domain in support for the publication "Internalization of therapeutic antibodies into Dendritic cells as a risk factor for immunogenicity" by Michel Siegel, Anna-Lena Bolender, Axel Ducret, Johannes Fraidling, Katha...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-08-26 | MSV000095695 | MassIVE
The saprophytic mold Aspergillus fumigatus produces small (2-3 µm) airborne spores (conidia) that can reach the lung alveoli upon inhalation. There, they encounter the surfactant-rich environment of the alveolar epithelium and initiate swelling and germination. Primary alveolar macrophages are essen...
ORGANISM(S): Homo sapiens 
The appended raw files, csv files and other documents were deposited into the public domain in support for the publication "Development and characterization of a dendritic cell internalization assay contributing to the immunogenicity risk evaluation of biotherapeutics" by Michel Siegel, Aman Padamse...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-08-12 | MSV000095578 | MassIVE
Generation of human monocyte-derived DCs Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll density centrifugation from buffy coats or whole blood of healthy donors and resuspended in serum-free medium (CellGro, CellGenix GmbH, Freiburg, Germany), supplemented with penicillin (50U/ml)...
ORGANISM(S): Homo sapiens 
To study the effect of stress on macrophages due to Toxoplasma, we stimulated murine bone marrow-derived macrophages (BMDMs) with IFN-γ (no-stimulate control) and infected them with the apicomplexan parasite Toxoplasma gondii. scRNA-Seq (10X Chromium genomics ) was performed to understand the change...
ORGANISM(S): Mus musculus 
We generated a systematic, quantitative and deep proteome and transcriptome abundance atlas from 29 paired healthy human to serve as a molecular baseline to study human biology.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-02 | MSV000083508 | MassIVE
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