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ProteomicsIdentification of GSDME-interacting proteins in HK-2 cells by immunoprecipitation-coupled LC-MS/MS analysis
This project aims to identify GSDME-associated proteins in human renal tubular epithelial (HK-2) cells using immunoprecipitation followed by LC-MS/MS. HK-2 cells overexpressing Flag-tagged GSDME were lysed, and protein complexes were enriched using anti-Flag antibody and Protein A/G magnetic beads. ...
ORGANISM(S): Homo Sapiens 
2025-04-12 | PXD062902 |
ProteomicsA proximity labeling map of SARS-CoV-2 and human
The global epidemic caused by the coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in the infection of over 200 million people. To extend the knowledge of interactions between SARS-CoV-2 and humans, we systematically investigate the interactome of 29 viral protei...
ORGANISM(S): Homo Sapiens 
2022-01-06 | PXD022086 |
ProteomicsMass spectrometry of HCT116 cells
HCT116 cells were transfected with flag-pcDNA3.1 plasmids or flag-MST3 plasmids. Whole-cell extracts were prepared with a lysis buffer, lysed on ice for 10 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The anti-Flag antibody and protein A/G beads were added and...
ORGANISM(S): Homo Sapiens 
2024-03-09 | PXD050513 |
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