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ProteomicsHspB8 controls molecular aging of FUS condensates by domain-specific protein interactions Part 2
Biomolecular condensates formed by phase separation offer a confined space where protein-protein interactions (PPIs) facilitate distinct biochemical reactions. As their aberrant behavior is associated with disease, it is crucial to understand the molecular organization of PPIs within condensates. Us...
ORGANISM(S): Homo sapiens (Human) 
2021-10-13 | PXD021115 | Pride
Molecular condensates Rrm Protein dynamics Fus Structural proteomics Chaperone Time-resolved quantitative xl-ms Aging 
ProteomicsCross-linking mass spectrometry analysis of trimeric complex of CRM1, SNP1 and RanGTP cross-linked with BS3
An X-ray crystallography structure of the trimeric nuclear export complex of CRM1, SNP1 and RanGTP is available on the PDB database with the ID 3GJX. To get an XL-MS dataset of a clean sample of a stable protein complex for benchmarking purposes, another sample from the protein expression, purificat...
ORGANISM(S): Escherichia coli 
2020-10-20 | PXD014359 | Pride
Technical dataset 
UnknownA comprehensive landscape of 60S ribosome biogenesis factors.
Not available
S-EPMC8884084 | biostudies-literature
ProteomicsValidation of filter aided sample preparation (FASP) approach for quantitative plant shotgun proteomics
Gel-free LC-based shotgun proteomics represents the current gold standard of proteome analysis due to its outstanding throughput, analytical resolution and reproducibility. Thereby, the efficiency of sample preparation, i.e., protein isolation, solubilization and proteolysis, directly affects the co...
ORGANISM(S): Bos taurus (Bovine) Pisum sativum (Garden pea) Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2023-03-11 | PXD025897 | Pride
Plant proteomics Filter aided sample preparation (fasp) Shotgun proteomics Lc-ms Phenol extraction Label-free quantification Detergent-assisted proteolysis Sodium dodecyl sulfate 
ProteomicsSpatial, Quantitative and Functional Deconstruction of Virus and Host Protein Interactions Inside Intact Cytomegalovirus Particles
Viral infections involve packaging of the viral genome and proteins into virions. Knowing virion composition and structure is critical to understand viral pathogenesis. However, an integrated picture of virion proteome organization of large viruses, such as Herpesviruses, is lacking. Here we use cro...
ORGANISM(S): Homo sapiens (Human) Cytomegalovirus Human betaherpesvirus 5 
2023-06-14 | PXD031911 | Pride
Cross-linking Virion Herpesvirus 
ProteomicsDeciphering changes to human platelet proteome and degradome during storage before transfusion
Proteases, and specifically metalloproteinases, have been linked to the loss of platelet function during storage before transfusion, albeit the mechanism remains unknown. We used a dedicated N-terminomics technique, multiplex iTRAQ-TAILS (Terminal Amine Isotope Labeling of Substrates), to characteri...
ORGANISM(S): Homo sapiens (Human) 
2014-10-24 | PXD000906 | Pride
Platelets Metalloproteinases Degradome Proteases Tails Human Ls-msms 
TranscriptomicsMeiosis timecourse Rap1-depletion expression profile
To investigate the impact of Rap1 binding on nearby gene expression during the transition from vegetative growth to meiosis, we measured RNA abundance in cells depleted (plus doxycycline) or not depleted (minus doxycycline) of Rap1. RNA abundance was measured in depleted and non-depleted condition i...
ORGANISM(S): Saccharomyces cerevisiae SK1 
2016-09-01 | E-GEOD-75259 | biostudies-arrayexpress
TranscriptomicsMeiosis timecourse Rap1-depletion Rap1 ChIP-chip
To confirm that Rap1-depletion resulted in decreased binding of Rap1 to its target sites, we performed compared Rap1 ChIP-chip experiments in respiratory cells (pre-meiotic/YPA 20 hours) depleted (plus doxycycline for 12 hours) or not depleted (minus doxycycline) of Rap1. There is growing recognitio...
ORGANISM(S): Saccharomyces cerevisiae SK1 
2016-09-01 | E-GEOD-75260 | biostudies-arrayexpress
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