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ProteomicsTINC - a method to dissect transcriptional complexes at single locus resolution - reveals novel Nanog regulators in mouse embryonic stem cells
Cellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in genome-wide epigenome profiling techniques have significantly increased our understanding of cell-specific utilization of REs. However, it remai...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-14 | PXD022088 | Pride
Nanog Cellular identity Stem cells Regulatory elements Tinc 
TranscriptomicsTINC - a method to dissect transcriptional complexes at single locus resolution - reveals novel Nanog regulators in mouse embryonic stem cells
We developed a novel epigenetic method termed TINC: TALE-mediated Isolation of Nuclear Chromatin. Using TINC we interrogated the protein complex formed at the Nanog promoter in embryonic stem cells (ESCs) and identified many known interactors as well as numerous previously unknown complex members, i...
ORGANISM(S): Mus musculus 
2020-11-05 | GSE160816 | GEO
GenomicsTINC - a method to dissect transcriptional complexes at single locus resolution - reveals novel Nanog regulators in mouse embryonic stem cells
TINC - a method to dissect transcriptional complexes at single locus resolution - reveals novel Nanog regulators in mouse embryonic stem cells
PRJNA674588 | ENA
UnknownTINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter.
Not available
S-EPMC7724517 | biostudies-literature
TranscriptomicsRNA-seq of FACS purified cardiomyocytes from Drosophila melanogaster embryos at stage 13-16.
To analyse the transcriptome landscape of differentiating cardiomyocytes, GFP labeled cardiac cells were purified by fluorescent activated cell sorting using the TinC*>GFP transgenic line, and expression profile analysed by deep sequencing.
ORGANISM(S): Drosophila melanogaster 
2017-01-17 | E-MTAB-4763 | biostudies-arrayexpress
UnknownDevelopment of a validated PROM set for children with tinnitus using the Patient-Reported Outcomes Measurement Information System - Protocol for the 'Tinnitus in Children' (TinC) study.
Not available
S-EPMC12530566 | biostudies-literature
GenomicsRNA-seq of FACS purified cardiomyocytes from Drosophila melanogaster embryos at stage 13-16.
RNA-seq of FACS purified cardiomyocytes from Drosophila melanogaster embryos at stage 13-16.
PRJEB19027 | ENA
GenomicsBiTS Chromatin Immunoprecipitation of H3K4me3 and H3K27ac in Drosophila melanogaster differentiating cardiomyocytes
Here we improved BiTS-ChIP (Bonn et al, Nature Protocols 7, 978-994 (2012)) to identify active enhancer and promoter elements genome wide in the 104 cardiomyocytes that constitute the Drosophila heart tube and represents only ~0.5% of the total cell content of the embryo. A transgenic Drosophila str...
ORGANISM(S): Drosophila melanogaster 
2017-01-10 | E-MTAB-4584 | biostudies-arrayexpress
GenomicsTime course Pho/dSfmbt Chromatin Immunoprecipitation on Drosophila melanogaster embryos during embryogenesis
Here we improved BiTS-ChIP (Bonn et al, Nature Protocols 7, 978-994 (2012)) to identify active enhancer and promoter elements genome wide in the 104 cardiomyocytes that constitute the Drosophila heart tube and represents only ~0.5% of the total cell content of the embryo. A transgenic Drosophila str...
ORGANISM(S): Drosophila melanogaster 
2017-04-11 | E-MTAB-4585 | biostudies-arrayexpress
UnknownEffects of Spherical and Rod-like Gold Nanoparticles on the Reactivity of Human Peripheral Blood Leukocytes.
Not available
S-EPMC10885998 | biostudies-literature
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