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ProteomicsHuman pancreatic endocrine cells, LC-MS3
FACS isolated human alpha and beta cells were analyzed by TMT 11-plex labeling, Off-line basic pH reversed phase fractionation and LC-MS3 on an Orbitrap Fusion mass spectrometer.
ORGANISM(S): Homo sapiens (Human) 
2019-02-15 | PXD011933 | Pride
Human Endocrine cells Tmt 11-plex Lc-ms3 
ProteomicsEnhanced sample multiplexing-based targeted proteomics with intelligent data acquisition
Targeted proteomics has been playing an increasingly important role in hypothesis-driven protein research and clinical bi-omarker discovery. We have previously created a workflow, Tomahto, to enable real-time targeted pathway proteomics assays using two-dimensional multiplexing technology. Coupled w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-08-14 | MSV000095604 | MassIVE
ProteomicsSpatial Proteomic Analysis of Isogenic Metastatic Colorectal Cancer Cells Reveals Key Dysregulated Proteins Associated with Lymph Node, Liver and Lung Metastasis
Liver, lung and lymph nodes are the most common metatastic sites of colorectal cancer (CRC) cells. Here, we aimed to analyze by quantitative spatial proteomics the isogenic KM12 cell system (non-metastatic KM12C cells, liver metastatic KM12SM cells and liver and lung metastatic KM12L4a cells), and t...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD030671 | Pride
Spatial proteomics Colorectal cancer Tmt 11-plex Cells 
ProteomicsPhosphoproteomics analysis of Selinexor response in AML ex vivo patient samples and cell lines
We used mass spectrometry-based proteomics to perform a phosphoproteomics analysis of selinexor-treated ex vivo AML patient samples (n=20) and 4 AML cell lines (PL-21, NOMO-1, GDM-1 and MV4-11). For quantitative phosphoproteomics, we used a tandem mass tag (TMT)-based approach. Conditions for the TM...
ORGANISM(S): Homo sapiens (Human) 
2022-08-07 | PXD017660 | Pride
Lc-msms Selinexor Human Aml 
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsProteomics-based approach reveals the involvement of SERPINB9 in recurrent and relapsed multiple myeloma
Multiple myeloma (MM) is a common hematological malignancy with poorly understood recurrence and relapse mechanisms. Notably, bortezomib resistance leading to relapse makes MM treatment significantly challenging. To clarify the drug resistance mechanism, we employed a quantitative proteomics approac...
ORGANISM(S): Homo sapiens (Human) 
2021-03-03 | PXD022182 | Pride
Multiple myeloma  recurrence and relapse  proteomics  parallel reaction monitoring  serpinb9 
Proteomicsn Efficient, Amine-Specific, and Cost-Effective Method for TMT 6/11-plex Labeling Improves the Proteome Coverage, Quantitative Accuracy and Precision
Tandem mass tags (TMT) are among the most popular proteomics quantification techniques due to its ability to simulta-neously quantify multiple samples in a single experiment. The tags can be easily added onto the primary amine groups of peptides and proteins through chemical reactions. Although TMT ...
ORGANISM(S): Homo Sapiens 
2023-11-17 | PXD047052 |
ProteomicsIdentifying the proteome and phosphoproteome regulated by the Mck1 kinase in response to glucose starvation
Using the 11-plex TMT labelling kit, the project intended to identify the proteins and phosphoproteins in the S. cerevisiae proteome that are differentially regulated by the Mck1 kinase during the transition from exponential growth to the post-diauxic shift phase.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-05-26 | PXD055585 | Pride
Mck1 Post-diauxic shift phase Proteome 
ProteomicsCD3/CD28 signaling, Jurkat E6.1, time course 27 mins
Fe-IMAC phosphoproteomics using TMT 11plex for quant, of Jurkat T cells stimulated with CD3 and CD28 agonist antibodies for 0, 3, 9, or 27 minutes.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000092965 | MassIVE
ProteomicsTemporal Expression Profiling of Plasma Proteins in Type 1 Diabetes Progression
A longitudinal proteomic profiling of human plasma using TMT-10plex-based LC-MS/MS analysis was performed to track temporal proteomic changes of T1D patients (n = 11) across 9 serial time points, spanning the period of T1D natural progression, in comparison with those of the matching healthy control...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000084341 | MassIVE
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