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ProteomicsTMT labeling for the masses: A robust and cost-efficient, in-solution labeling strategy
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quan...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-14 | PXD012703 | Pride
Stable isotope labeling Tandem mass tags Nhs ester chemistry Labeling efficiency 
UnknownAssessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH.
Not available
S-EPMC8154127 | biostudies-literature
UnknownA benchmarking protocol for intact protein-level Tandem Mass Tag (TMT) labeling for quantitative top-down proteomics.
Not available
S-EPMC9587358 | biostudies-literature
UnknownGlobal quantitative proteomic analysis profiles of host protein expression in response to enterovirus A71 infection in bronchial epithelial cells based on tandem mass tag (TMT) peptide labeling coupled with LC-MS/MS uncovers the key role of proteasome in virus replication.
Not available
S-EPMC10194169 | biostudies-literature
UnknownComparison of protein expression ratios observed by sixplex and duplex TMT labeling method.
Not available
S-EPMC3751587 | biostudies-literature
ProteomicsExtended multiplexing of TMT labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue
Age-related and/or high caloric intake driven changes in visceral adipose tissue contribute to the development of diabetes and cardiovascular disease. The lack of reliable, high-throughput methods to analyze the adipose tissue protein composition has limited our understanding of the protein network...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-07-03 | MSV000082569 | MassIVE
ProteomicsMultiple sclerosis biomarker discovery in cerebrospinal fluid using glycopeptide enrichment, TMT labeling and LC-MS/MS
Multiple sclerosis biomarker discovery in pooled cerebrospinal fluid (CSF) using glycopeptide enrichment, TMT labeling and LC-MS/MS. Comparing 3 pools of CSF from relapsing-remitting MS (RRMS) patients to 3 pools of CSF from patients with other neurological diseases (OND). To reveal protein groups a...
ORGANISM(S): Homo sapiens (Human) 
2016-10-13 | PXD004540 | Pride
Human Cerebrospinal fluid Tmt Discovery Glycopeptide enrichment Lc-ms/ms 
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
ProteomicsIsobaric peptide labeling on digital microfluidics for quantitative proteomics of low cell numbers is facilitated by low critical micellar concentration detergents
We developed protocols for isobaric TMT labeling on digital microfluidics (DMF) devices enabling the quantitative proteome analysis of approximately 25 mammalian cells per channel by subsequent bottom-up proteome analysis by LC-MS. This comprised identification of a compatible detergent for digestio...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD023226 | Pride
Digital microfluidics Detergents Cmc Tmt labeling Sp3 
UnknownMultidimensional Separation Using HILIC and SCX Pre-fractionation for RP LC-MS/MS Platform with Automated Exclusion List-based MS Data Acquisition with Increased Protein Quantification.
Not available
S-EPMC4720167 | biostudies-literature
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