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TranscriptomicsTranscription profiling by array of human mammary epithelial cells (HMEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs
HMEC cultures were left untreated or stimulated for 5h with 2 ng/ml TNF. Comparison of the gene expression profiles revealed the TNF-mediated gene expression changes.
ORGANISM(S): Homo sapiens 
2007-11-01 | E-GEOD-2638 | biostudies-arrayexpress
TranscriptomicsWhole blood transcriptional profiling of healthy volunteers and rheumatoid arthritis patients treated with methotrexate or anti-TNF therapy after ex vivo whole blood stimulation with TNF
We tested the hypothesis that anti-tumor necrosis factor (TNF) therapy reduces TNF-inducible gene expression in blood. Whole peripheral blood from healthy volunteers and rheumatoid arthritis patients (treated with the monoclonal anti-TNF antibody adalimumab, the soluble TNF receptor etanercept or th...
ORGANISM(S): Homo sapiens 
2017-08-04 | E-MTAB-5094 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human umbilical vein endothelial cells (HUVEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs
HUVEC were left untreated or stimulated for 5h with 2 ng/ml TNF. Comparsion of the gene profiles revealed TNF-mediated gene expression changes in HUVEC.
ORGANISM(S): Homo sapiens 
2007-11-01 | E-GEOD-2639 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of inducible GATA6L-overexpressing HT-29 cells stimulated with TNF
The objective of this study was to investigate the role of GATA6L with or without TNF stimulation. Microarrays were used to capture the transcriptional response to GATA6L overpexression at intermediate times after TNF stimulation. Cells were induced for GATA6L overexpression and evaluated at two hou...
ORGANISM(S): Homo sapiens 
2016-07-25 | E-GEOD-72079 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of HT-29 cells stimulated with pairwise combinations of TNF, EGF, and insulin
The objective of this study was to investigate the link between signaling-network activation and transcriptional regulation downstream of tumor necrosis factor (TNF), epidermal growth factor (EGF), and insulin. Microarrays were used to capture the transcriptional responses to TNF, EGF, and insulin a...
ORGANISM(S): Homo sapiens 
2016-07-25 | E-GEOD-69639 | biostudies-arrayexpress
TranscriptomicsTranscriptional response of LPS-stimulated monocyte-derived macrophages in the presence or absence of the anti-TNF agonist Etanercept
We measured TNFα activity on the transcriptional level by identifying TNFα-inducible genes in human blood monocyte-derived macrophages (MDM). On the basis that TNFα does not act in isolation, we used bacterial lipopolysaccharide (LPS) as a prototypic model of a stimulus to invoke secretion of a w...
ORGANISM(S): Homo sapiens 
2017-08-04 | E-MTAB-5095 | biostudies-arrayexpress
ProteomicsMCF-7 phosphoproteome LC-MS/MS
MCF-7 cells were stimulated with TNF-alpha in order to identify IKKb substrates. conditions: TNF alpha stimulation TNF alpha stimulation + SC-514 IKK (kinase dead mutant) + TNF alpha stimulation IKK(WT) + TNF alpha stimulation basal Already validated IKK substrates were used to train random fores...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD001873 | Pride
Mcf-7 Phosphoproteome Tnf stimulation Ikkb 
TranscriptomicsModulation of the TNF-induced macrophage response by synovial fibroblasts
Here we explored how the human macrophage response to tumor necrosis factor (TNF) is regulated by human synovial fibroblasts, the representative stromal cell type in the synovial lining of joints that become activated during inflammatory arthritis. Genome-wide transcriptome analysis (RNAseq) showed ...
ORGANISM(S): Homo sapiens 
2014-08-04 | E-GEOD-57723 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of siRNA transfected HEK293T cells untreated or treated with TNF-alpha to study the effect of siRNA-mediated knockdown of different genes
HEK293T cells were treated with TNF-alpha for different periods of time to study the effect of siRNA-mediated knockdown of different genes (negative control: Renilla luciferase; positive controls: TNFRSF1A, RelA; gene of interest: CASP4) on TNF-induced gene expression
ORGANISM(S): Homo sapiens 
2012-07-06 | E-MTAB-900 | biostudies-arrayexpress
TranscriptomicsExpression data of HUVEC cells after TNF-alpha treatment
Tumor necrosis factor a (TNF-aplha) is a potent cytokine that orchestrates the inflammatory response by sequentially activating the expression of many genes in cultured human umbilical vein cells (HUVECs). We apply samples collected every 7.5 min to arrays and monitor the growth of several long tran...
ORGANISM(S): Homo sapiens 
2007-09-19 | E-GEOD-9036 | biostudies-arrayexpress
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