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MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
TranscriptomicsLysate affects on keratinocyte scratch assays
The aim of this study was to provide proof of principle as to whether probiotic bacteria or their extracts, could stimulate cutaneous wound healing. To this end, we have used a keratinocyte monolayer scratch assay which assesses one important aspect of wound healing, re-epithelialization. Primary hu...
ORGANISM(S): Homo sapiens 
2016-03-23 | E-MTAB-3485 | biostudies-arrayexpress
TranscriptomicsGene expression in C57BL/6 mouse lungs after exposure to aerosolized NTHi lysate
Pneumonia is a serious problem worldwide. We recently demonstrated that innate defense mechanisms of the lung are highly inducible against pneumococcal pneumonia. To determine the breadth of protection conferred by stimulation of lung mucosal innate immunity, and to identify cells and signaling pa...
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-13740 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of leukemia samples prepared using different RNA isolation procedures
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. Fo...
ORGANISM(S): Homo sapiens 
2008-11-06 | E-GEOD-7757 | biostudies-arrayexpress
MetabolomicsMetabolic Dynamics in Escherichia coli-Based Cell-Free Systems
The field of metabolic engineering has yielded remarkable accomplishments in using cells to produce valuable molecules, and cell-free expression (CFE) systems have the potential to push the field even further. However, CFE systems still face some outstanding challenges, including endogenous metaboli...
2023-02-22 | MTBLS2630 | MetaboLights
ProteomicsE. coli S30 lysate proteome - The E
Protein production using processed cell lysates, defined precursor cocktails and target specific additives has become a core technology in the field of synthetic biology. Due to their open nature, these artificial systems are excellent to produce difficult proteins such as toxins or membrane protein...
ORGANISM(S): Escherichia coli 
2017-09-29 | PXD004646 | Pride
Qualitative and quantititative proteomics Heatshock lysate E. coli a19 S30 lysate 
TranscriptomicsTranscription profiling of rat perinatal lung polysomal and total RNA
During the peri-partum period, the lung must respond to many factors with potential to impact protein synthesis via regulation of translation initiation. Microarray analysis of polysomal versus total RNA from fetal day (FD) 19, FD22 and postnatal day 1 (P1) rat lungs was used to identify genes whos...
ORGANISM(S): Rattus norvegicus 
2009-07-11 | E-GEOD-12153 | biostudies-arrayexpress
TranscriptomicsThe effect of miRNA overexpression on gene expression profile of MCF-7 cells
To identify microRNAs impacting estrogen receptor ERα expression in breast cancer, we have screened ER-positive breast cancer cells with a library of pre-miRs, and systematically monitored the ERα expression by protein lysate microarrays. There was a significant enrichment of the in silico predict...
ORGANISM(S): Homo sapiens 
2010-04-09 | E-GEOD-14847 | biostudies-arrayexpress
no omics definedmRNA expression profiling of human mesenchymal stromal cell samples grown in vitro under different conditions (foetal calf serum (FCS) based medium versus human platelet lysate (PL) based medium)
Genetic profiling after stem cell culturing. Culturing cells from normal donors, grown in 2 culture media
ORGANISM(S): Homo sapiens 
2023-03-01 | E-MTAB-12272 | biostudies-arrayexpress
ProteomicsOpisthorchis felineus excretory-secretory product (ESP) and lysate proteomics by GeLC-MS / MS
A significant number of pathological conditions, accompanied by chronic non-healing wounds, demands searching for new modern therapeutic approaches. Well-documented ability of O. felineus to initiate extracellular matrix (ECM) remodeling and liver epithelium regeneration suggests that its bioactive ...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD037991 | Pride
Opisthorchis felineus Sds-page Excretory-secretory product Lysate Lc-ms/ms 
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