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Identification of ubiquitination sites on my protein of interest (TRIM52) through NiNTA pull-down of TRIM52 modified by his-ubiquitin.
ORGANISM(S): Homo sapiens (Human) 
2025-03-25 | PXD051295 | Pride
All constructs were expressed in RKO cells (human rectal carcinoma cell line). A fusion construct of TurboID (mutated BirA* from E. Coli) and green fluorescent protein (GFP) or human TRIM52. Cells were stimulated with Epoxomicin (proteasome inhibitor) or left untreated. Biotinylated proteins were e...
ORGANISM(S): Homo sapiens (Human) 
2025-03-25 | PXD051272 | Pride
Global transcriptional changes in U87MG glioblastoma cells upon shRNA-mediated TRIM52 knockdown
shRNA-mediated ablation of the RING-finger protein TRIM52 from multiple glioblastoma cell lines reduces proliferation and tumorigenesis. To identify gene signatures underlying this phenomenon, transcritional profile of TRIM52 knockdown cells was compared to control cells. Upon TRIM52 ablation, we fi...
ORGANISM(S): Homo sapiens 
2018-04-01 | GSE107932 | GEO
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