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We performed a TMT-based quantitative proteomic analysis of SAHA-perturbed human HeLa cells using advanced RTS platform on Orbitrap Eclipse Tribrid MS system.
ORGANISM(S): Homo Sapiens (human) 
EGF-induced regulation of Gamma-enolase protein complexes was analyzed using anti-Gamma-enolase monoclonal antibodies designed to immunoreact with the C-terminus of human Gamma-enolase protein.
ORGANISM(S): Homo Sapiens (human) 
We performed a TMT-based quantitative proteomic analysis of EGF-treated human cancer cells in the presence/absence of Torin 1 using advanced RTS platform on Orbitrap Eclipse Tribrid MS system.
ORGANISM(S): Homo Sapiens (human) 
PDZD8-TurboID knocked-in HeLa cells were plated into a 15 cm dish and cultured two overnight. The cells were treated with Biotin at the final concentration of 50 μM, and incubated for 6 hours. The cells were washed twice with ice-cold Hepes-saline and lysed in 6 M guanidine-HCl containing 100 mM HEP...
ORGANISM(S): Homo Sapiens (human) 
Pdzd8-3xHA knock-in mice and control littermates at postnatal 10 days were put to sleep using medetomidine hydrochloride, midazolam and butorphanol. Pups were then put on the ice for 5 minutes and exsanguinated by terminal intracardial perfusion with ice-cold 2% paraformaldehyde in PBS. The neocorte...
ORGANISM(S): Mus Musculus (mouse) 
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