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Proteomicsphosphoproteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice
We obtained the profiles of neuronal phosphoproteome after cerebral ischemia and reperfusion by isolating mice hippocampus. Hippocampus combined from either nine sham or nine focal cerebral ischemia 1.5 h and reperfusion 24 h (IR) mice were lysed, digested, labeled with different TMT tags, then pool...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-15 | PXD033851 | Pride
Phosphoproteome  hippocampus  cerebral ischemia and reperfusion 
ProteomicsProteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice
We obtained the profiles of neuronal proteome after cerebral ischemia and reperfusion by isolating mice hippocampus. Hippocampus combined from either nine sham or nine focal cerebral ischemia 1.5 h and reperfusion 24 h (IR) mice were lysed, digested, labeled with different TMT tags, then pooled and ...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-15 | PXD033938 | Pride
Proteome  hippocampus  cerebral ischemia and reperfusion 
TranscriptomicsTU-tagging: cell type specific RNA isolation from intact complex tissues
We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to i...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-15445 | biostudies-arrayexpress
ProteomicsProteome and phosphoproteome of hippocampus after focal cerebral ischemia 2 h in mice
We obtained the profiles of neuronal phosphoproteome after cerebral ischemia onset by isolating mice hippocampus. Hippocampus combined from either ten sham or ten focal cerebral ischemia 2 h mice were lysed, digested, labeled with different TMT tags, then pooled and analyzed by LC/LC-MS/MS. Five per...
ORGANISM(S): Mus musculus (Mouse) 
2022-03-14 | PXD027145 | Pride
Proteome   phosphoproteome  hippocampus  cerebral ischemia 
UnknownMycoplasma hyopneumoniae evades complement activation by binding to factor H via elongation factor thermo unstable (EF-Tu).
Not available
S-EPMC7549910 | biostudies-literature
ProteomicsThe Shewanella oneidensis Fic enzyme SoFic targets theswitch-I region of EF-Tu for AMPylation
Fic enzymes mediate diverse post-translational modifications, including adeno-sine monophosphate (AMP) transfer and removal, referred to as AMPylationand deAMPylation, respectively. We identified the prokaryotic translationelongation factor Tu (EF-Tu) as an AMPylation target of the Fic enzymeSoFic. ...
ORGANISM(S): Escherichia coli 
2026-09-18 | PXD071331 | Pride
Structural regulation Ampylation Ef-tu Fic enzymes Deampylation 
GenomicsDanio rerio strain:TU
Danio rerio strain:TU Raw sequence reads
PRJNA612371 | ENA
TranscriptomicsRadiation exposure of mouse embryonic brain - 2 h, 24 h post-irradiation
The mammalian brain is especially sensitive to ionizing radiation during development, as shown by the increased occurrence of mental retardation and small head size in children who were in utero exposed to ionizing radiation after the atomic bombings of Hiroshima and Nagasaki. These effects of prena...
ORGANISM(S): Mus musculus 
2015-07-01 | E-MTAB-2720 | biostudies-arrayexpress
ProteomicsCSF levels of Elongation Factor Tu is associated with increased mortality in human Streptococcus pneumoniae meningitis
Background Mortality from bacterial meningitis, predominately caused by Streptococcus pneumoniae, exceeds 50% in low and middle income countries in sub-Saharan Africa with high HIV prevalence. Underlying causes of high mortality are poorly understood. We examined the host and pathogen proteome in a...
ORGANISM(S): Homo sapiens (Human) 
2020-11-09 | PXD021268 | Pride
Cerebrospinal fluid Proteomics Hiv Mortality Streptococcus pneumoniae 
TranscriptomicsGenomewide measurements of neural-specific mRNA decay in Drosophila embryos
Neural-specific mRNA decay measurements by TU-Decay technique in control and Pumilio knockdown embryos These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Neural-specific RNA purification was achieved using prospero-GAL4 drivin...
ORGANISM(S): Drosophila melanogaster 
2015-04-02 | E-GEOD-67512 | biostudies-arrayexpress
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