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Chlamydomonas wild type CC 4533 and lpa2 mutant were grown in TAP medium at 25°C and ~30 µmol photons m^-2 s^-1 on a rotary shaker. Protein complexes of three wild type (wt) and three lpa2 (mut) samples were separated by BN-PAGE and cut into 36 slices. Subsequent mass spectrometry enables the analys...
ORGANISM(S): Chlamydomonas reinhardtii 
2021-09-28 | PXD023443 | Pride
Samples were reduced in loading buffer and separated by SDS-PAGE for 3-4 min followed by in-gel digestion with trypsin. Digests were analyzed by LC-MS/MS using a Waters nanoACQUITY interfaced to a Thermo Fusion Lumos MS. The LC separation 90 min gradient of 5-30% MeCN in a trapping configurations w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-12-30 | MSV000086651 | MassIVE
Chlamydomonas wild type CC 4533 and psb28 mutant were grown in TAP medium at 25°C and ~30 µmol photons m^-2 s^-1 on a rotary shaker. Protein complexes of three wild type (wt) and three psb28 (mut) samples were separated by BN-PAGE and cut into 36 slices. Subsequent mass spectrometry enables the anal...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-05-08 | PXD023478 | Pride
Using gene expression profiling we characterize the global effect of p53 on the TLR5-mediated transcription in MCF7 cells. We found that combined activation of p53 and TLR5 pathways synergistically increases expression of over 200 genes, mostly associated with immunity and inflammation. The synergy ...
ORGANISM(S): Homo sapiens 
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