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AGO protein immunoprecipitation was combined with high-throughput sequencing of associated small RNAs. AGO2, AGO10, and to a lesser extent AGO1 were shown to associate with siRNAs derived from silencing suppressor (HC-Pro)-deficient TuMV-AS9, but not with siRNAs derived from wild-type TuMV. Co-immun...
ORGANISM(S): Arabidopsis thaliana 
Successful subversion of translation initiation factors 4E and 4G determines the infection success of potyviruses, the largest group of viruses affecting plants. Functional redundancy among these factors allows to engineer resistances through their genetic inactivation, however recent findings indic...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-10-26 | PXD036336 | Pride
Arabidopsis ecotype Col-0 expressing RPL18 fused to His & Flag epitopes (RPL18-HF) under 35S control was used to discern the effects of Turnip mosaic virus (TuMV) infection on host translation initiation. Plants were grown in LC-1 soil in 12 hour days and infected by TuMV-GFP or mock-inoculated jus...
ORGANISM(S): Arabidopsis thaliana 
Individual miRNA profile was identified for two Col-0 WT replicates and two Col-0 TuMV infected plant replicate samples, with 12-15 plants in each replicate.Comparison was done based on the microRNA expression profile in both biological replicates for infected and uninfected plant samples . 12-15 pl...
ORGANISM(S): Arabidopsis thaliana 
Arabidopsis ecotype Col-0 expressing RPL18 fused to His & Flag epitopes (RPL18-HF) under 35S control was used to discern the effects of Turnip mosaic virus (TuMV) infection on host translation initiation. Plants were grown in LC-1 soil in 12 hour days and infected by TuMV-GFP or mock-inoculated just...
ORGANISM(S): Arabidopsis thaliana 
2011-03-11 | GSE20278 | GEO
sRNAseq from Arabidopsis leaves non-infected and infected with Turnip mosaic virus (TuMV)
Genomics
TuMV RNA-seq
AtHsp70-14 and -15 (At1g79930 and At1g79920) are two closely related isoforms of the Hsp110/SSE subfamily of the HSP70 chaperones in Arabidopsis thaliana. By analysing T-DNA insertion and amiRNA-AtHsp70-14/15 lines, we could demonstrate an essential function of AtHsp70-15 for plant growth. AtHsp70-1...
ORGANISM(S): Arabidopsis thaliana 
Genomics
TuMV-infected Arabidopsis
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