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TranscriptomicsPromoter directionality is controlled by U1 snRNP and polyadenylation signals
Transcription of the mammalian genome is pervasive, but productive transcription outside of protein-coding genes is limited by unknown mechanisms. In particular, although RNA polymerase II (RNAPII) initiates divergently from most active gene promoters, productive elongation occurs primarily in the s...
ORGANISM(S): Mus musculus 
2013-06-28 | E-GEOD-46433 | biostudies-arrayexpress
TranscriptomicsExon arrays from HeLa cells transfected with GFP-ZRANB2 vector
Human ZRANB2 mRNA is alternatively spliced to give two variants with different 3’ ends. Both isoforms are present in the nucleus of human cells. ZRANB2 binds to mRNA, as well as the essential splicing factors U170K and U2AF35, and the novel splicing component SFRS17A (formerly known as XE7). It has ...
ORGANISM(S): Homo sapiens 
2012-12-15 | GSE32932 | GEO
TranscriptomicsiCLIP and NGS to map protein-RNA interactions for U1-snRNP proteins in Trypanosoma brucei
Individual-nucleotide resolution UV-crosslinking and immunoprecipitation (iCLIP) combined with high-throughput sequencing was performed to generate genome-wide binding maps of two U1-snRNP proteins: U1C and U1-70K in Trypanosoma brucei. 3 (2) biological replicates of U1C (U1-70K) -specific co-immuno...
ORGANISM(S): Trypanosoma brucei 
2014-05-16 | E-GEOD-43848 | biostudies-arrayexpress
TranscriptomicsExon arrays from HeLa cells transfected with GFP-ZRANB2 vector
Human ZRANB2 mRNA is alternatively spliced to give two variants with different 3M-bM-^@M-^Y ends. Both isoforms are present in the nucleus of human cells. ZRANB2 binds to mRNA, as well as the essential splicing factors U170K and U2AF35, and the novel splicing component SFRS17A (formerly known as XE7...
ORGANISM(S): Homo sapiens 
2012-12-15 | E-GEOD-32932 | biostudies-arrayexpress
TranscriptomicsRNA-seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70
It has been recently shown that SNRNP70, a major component of the spliceosome, as well as other splicing regulators are found in axons. To investigate the role of SNRNP70 in axons, we generated a zebrafish null mutant and found motor connectivity defects that can be partially rescued upon transgenic...
ORGANISM(S): Danio rerio 
2022-10-31 | E-MTAB-12301 | biostudies-arrayexpress
ProteomicsU1 Small Nuclear Ribonucleoprotein Complex and RNA Splicing Alterations in Alzheimer Disease
Comprehensive study of the human brain-insoluble proteome in AD by mass spectrometry. Mass spectrometry analysis of detergent insoluble fractions was processed based on our optimized LC-MS/MS protocol. Protein concentration was determined by bicinchoninic acid (BCA) assay (Thermo Scientific) using b...
ORGANISM(S): Homo sapiens (Human) 
2013-08-28 | PXD000067 | Pride
Human Splicing U170k Lc-ms/ms 
GenomicsSaccharomyces cerevisiae S288C
Saccharomyces cerevisiae S288C Raw sequence reads
PRJNA1229592 | ENA
TranscriptomicsGenome wide mapping of effects of U6atac knockdown on pre-mRNA splicing
This project looks into experimentally identifying all minor introns by knocking down the minor spliceosome's catalytic snRNP, U6atac. Knockdown of U6atac by antisense morpholino followed by examining mRNA splicing by RNA-seq
ORGANISM(S): Homo sapiens 
2013-08-01 | E-GEOD-48263 | biostudies-arrayexpress
TranscriptomicsIdentification of RNAs associated with the Caenorhabditis elegans SL1 snRNP SNA-1 protein by RIP-Seq
This study aimed to identify RNAs bound to the C. elegans SL1 snRNP SNA-1 protein through use of RNA immunoprecipitation sequencing. Three IP sample replicates and three control sample replicates were sequenced.
ORGANISM(S): Caenorhabditis elegans 
2022-06-07 | E-MTAB-10289 | biostudies-arrayexpress
TranscriptomicsAlternative Splicing in Erythropoiesis
Differentiating erythroid cells execute a unique gene expression program that insures synthesis of the appropriate proteome at each stage of maturation. Standard expression microarrays provide important insight into erythroid gene expression, but cannot detect qualitative changes in transcript struc...
ORGANISM(S): Homo sapiens 
2010-05-16 | E-GEOD-14588 | biostudies-arrayexpress
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