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We analyzed proteome of the anammox bacterium Kuenenia stuttgartiensis strain CSTR1 after separation by ultracentrifugation. Samples of the original cell extracts, membrane fraction, intermediate fraction (the liquid fraction that was directly above the membrane pellet), and cytosolic fraction were ...
ORGANISM(S): Candidatus Kuenenia stuttgartiensis 
2025-02-03 | PXD054936 | Pride
Extracellular vesicles (EVs) are central components of fungal biology, yet their isolation commonly relies on ultracentrifugation schemes originally developed for mammalian systems. In Cryptococcus, most EVs carry the capsular polysaccharide glucuronoxylomannan (GXM), raising the possibility that ve...
ORGANISM(S): Cryptococcus neoformans RCT29 
2026-06-29 | PXD076414 | Pride
Transcriptional analysis of THP-1 cells stimulated by bacterial extracellular vesicles enriched by ultracentrifugation and an ε-poly- L-lysine-based method
We propose an isolation strategy based on a broad-spectrum antimicrobial epsilon-poly-L–lysine (ε-PL) to precipitate bacterial extracellular vesicles (BEVs) at a relatively low centrifugal speed (10,000 × g). Compared to the standard ultracentrifugation (UC) strategy, our method can enrich BEVs from...
ORGANISM(S): Homo sapiens 
2022-08-02 | GSE207009 | GEO
MicroRNAs (miRNAs), small (18–22 nucleotides) non-coding RNAs, suppress the translation of target mRNAs by binding to their 3′ untranslated region. Evidence suggests that miRNAs are key regulators of several cellular processes, including angiogenesis. Recent findings indicate that Secreted miRNAs en...
ORGANISM(S): Homo sapiens 
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