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University of Greifswald Institute of Microbiology Department of Microbial Proteomics Felix-Hausdorff-Str.8 17489 Greifswald (1)
VIB Medical Biotechnology Center Department of Biochemistry, Ghent University A. Baertsoenkaai 3 B9000 Ghent Belgium (1)

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Genome-wide search for novel human uORFs and N-terminal protein extensions using ribosomal footprinting

So far, the annotation of translation initiation sites (TISs) has been based mostly upon bioinformatics rather than experimental evidence. We adapted ribosomal footprinting to puromycin-treated cells to generate a transcriptome-wide map of TISs in a human monocytic cell line. A neural network was tr...

ORGANISM(S): Homo sapiens

2012-08-08 | E-GEOD-39561 | biostudies-arrayexpress

eIF1 modulates the recognition of sub-optimal translation start sites and steers gene expression control mediated by uORFs.

Alternative translation initiation mechanisms such as leaky scanning and re-initiation potentiate the polycistronic nature of transcripts. By allowing for reprogrammed translation, these mechanisms can mediate biological responses to stress stimuli. We combined proteomics with ribosome profiling and...

ORGANISM(S): Homo sapiens (Human)

2017-09-13 | PXD004980 | Pride