The Upf1, Upf2 and Upf3 proteins cooperate to implement nonsense-mediated decay. We used DNA microarrays for profiling RNA expression levels of upf2 and upf3 mutants
All eukaryotes studied to date have the capacity to detect and degrade mRNAs harboring premature translation termination codons (PTCs) in a process called nonsense-mediated mRNA decay (NMD) (reviewed in Wagner E & Lykke-Andersen J, 2002). This surveillance system allows the cell to prevent the expre...
We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis upf1-1 and upf3-1 mutants. Keywords: untreated condition
Here, we performed total RNA-Seq in mutant strains of N. castellii lacking the NMD core factors Upf2 and Upf3, in order to identify lncRNAs and mRNAs that are regulated by NMD in this species.
To determine the effects of inactivation of both the nosense-mediated mRNA decay pathway and the general 5' to 3' decay pathway on yeast mRNA decay, we compared the expression profiles of the wild-type, xrn1, xrn1 upf1, xrn1 nmd2, and xrn1 upf3 strains.