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Exosomes/microvesicles (hereafter referred to as extracellular vesicles) were isolated from the ULF of day 14 cyclic and pregnant ewes using ExoQuick-TC. Extracellular vesicle RNA was pooled (n=4 per status) and analyzed for small RNAs by sequencing on the Ion Torrent PGM platform and analysis with ...
ORGANISM(S): Ovis aries 
The protein cargos of uterine extracellular vesicles isolated from uterine lavages, collected from pregnant mares (P; day 10, 11, 12 and 13 after insemination) and cyclic control mares (C; day 10 and 13 after insemination), was analyzed.
ORGANISM(S): Equus caballus (Horse) 
2022-10-15 | PXD032405 | Pride
Extracellular vesicles (EVs) have been identified in the uterine fluid in different species and pointed as key players in the embryo-maternal dialogue, maternal recognition of pregnancy (MRP) and establishment of pregnancy. However, little is known about the uterine EVs in the mare. Therefore, the p...
ORGANISM(S): Equus caballus (Horse) 
2021-09-09 | PXD022264 | Pride
Effect of uterine-derived extracellular vesicles on trophoblast cells
Uterine fluid (UF) samples were acquired from healthy cows, cows with clinical (CLE) and subclinical endometritis (SE). The extracellular vesicles (EVs) were isolated using size exclusion chromatography-based method. The enrichment of UF-EVs were confirmed with mass-spectrometry of a sample before a...
ORGANISM(S): Bos taurus (Bovine) 
2026-04-27 | PXD044219 | Pride
Majority of the patients with cervical cancer present with locally advanced cervical cancer (LACC), which is primarily treated using concurrent chemoradiation (CCRT) and lacks reliable predictive biomarkers. Extracellular vesicles (EVs) could potentially define the dynamic biological response to CCR...
ORGANISM(S): Homo Sapiens (human) 
Healthy cow uterine fluid (UF) was collected from synchronized cattle at day 0, 7 and 16 after ovulation. Extracellular vesicles (EVs) were isolated from UF. UF-EVs from same cattle on different timepoints were subjected to LC-MS/MS. Additionally, one sample before EV purification was subjected to L...
ORGANISM(S): Bos taurus (Bovine) 
2024-01-26 | PXD040201 | Pride
Bovine uterine fluid extracellular vesicles proteomic profile at luteal and follicular phases of the oestrous cycle
ORGANISM(S): Bos taurus (Bovine) 
2023-07-20 | PXD038002 | Pride
The present studies tested the hypothesis that the elongating ovine conceptus and uterus produces EVs with the potential to mediate conceptus-maternal communication during early pregnancy. In Study One, EVs were purified from uterine luminal fluid (ULF) of day 14 cyclic sheep. The EVs were fluoresce...
ORGANISM(S): Ovis aries 
Extracellular vesicles (EVs) released by endometrium into the uterine fluid are emerging as an important mediator of intercellular communication between mother and the embryo. Here, we show that these EVs are dynamically regulated for their protein composition during the natural menstrual cycle, an...
ORGANISM(S): Homo sapiens (Human) 
2021-03-18 | PXD020975 | Pride
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