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GenomicsUV-crosslinking and high throughput sequencing of cDNAs (CRAC) of the RNA chaperone Hfq in enterohaemorhaggic E. coli
UV-crosslinking and high throughput sequencing of cDNAs (CRAC) of the RNA chaperone Hfq in enterohaemorhaggic E. coli
PRJNA197291 | ENA
ProteomicsFly RBPome - Early embryogenesis is characterized by the maternal to zygotic transition (MTZ), in which maternally deposited messenger RNAs are translated and subsequently degraded while zygotic transcription begins
Early embryogenesis is characterized by the maternal to zygotic transition (MTZ), in which maternally deposited messenger RNAs are translated and subsequently degraded while zygotic transcription begins. Posttranscriptional gene regulation by RNA-binding proteins (RBPs) is a dominant force controlli...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-04-26 | PXD002992 | Pride
Fly 
ProteomicsEarly Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC
In this study, NAC was UV-crosslinked using the photo-crosslinking amino acid p-benzoyl-L-phenylalanine (pBPA) in order to determine specific interaction sites of the alphaNAC N-terminus in the native protein structure. Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2019-07-25 | PXD011995 | Pride
Nac Structural mass spectrometry Uv-crosslinking Q-xl-ms 
UnknownChemical crosslinking extends and complements UV crosslinking in analysis of RNA/DNA nucleic acid-protein interaction sites by mass spectrometry.
Not available
S-EPMC12359043 | biostudies-literature
GenomicsUPA-Seq: Prediction of functional lncRNAs using the sensitivities to UV-crosslinking
UPA-Seq: Prediction of functional lncRNAs using the sensitivities to UV-crosslinking
PRJNA472627 | ENA
TranscriptomicsUV-crosslinking and high throughput sequencing of cDNAs (CRAC) of the RNA chaperone Hfq in enterohaemorhaggic E. coli
UV-crosslinking and high througput sequencing of cDNAs (CRAC) was used to map the binding sites Hfq in enterohaemorhaggic E. coli (EHEC). Hfq was tagged with a His-FLAG dual affintiy tag and UV crosslinked after growth in the MEM-HEPES media essentailly as per Granneman et al (2009) PNAS. We additio...
ORGANISM(S): Escherichia coli O157:H7 str. Sakai Escherichia coli str. K-12 substr. MG1655 
2014-05-22 | GSE46118 | GEO
TranscriptomicsUV-crosslinking and high throughput sequencing of cDNAs (CRAC) of the RNA chaperone Hfq in enterohaemorhaggic E. coli
UV-crosslinking and high througput sequencing of cDNAs (CRAC) was used to map the binding sites Hfq in enterohaemorhaggic E. coli (EHEC). Hfq was tagged with a His-FLAG dual affintiy tag and UV crosslinked after growth in the MEM-HEPES media essentailly as per Granneman et al (2009) PNAS. We additio...
ORGANISM(S): Escherichia coli O157:H7 str. Sakai 
2014-05-22 | E-GEOD-46118 | biostudies-arrayexpress
ProteomicsFormaldehyde-crosslinking enables comprehensive profiling of in vivo RNA-interactome in UV-impermeable samples
Development of UV-crosslinking based RNA-interactome capture protocol have expanded the repertoire of RBPs. Apparent limitations of UV-crosslinking, however, have made the RBP profiling to be incomplete and inapplicable to many of the biological samples in physiological contexts. Here we developed f...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2020-11-25 | PXD010046 | Pride
Rbp formaldehyde 
GenomicsIdentification of RNA-RNA interactions in Bacillus Subtilis by in vivo UV crosslinking
Identification of RNA-RNA interactions in Bacillus Subtilis by in vivo UV crosslinking
PRJEB35178 | ENA
UnknownOptimisation of UV irradiation as a binding site conserving method for crosslinking collagen-based scaffolds.
Not available
S-EPMC4681752 | biostudies-literature
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