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TranscriptomicsRNAi knock down of NIPP1 and EZH2 in human prostate cell line PC-3 to investigate whether they regulate a common set of genes
NIPP1 and EZH2 contribute to the silencing of a common set of genes. Since both NIPP1 and EZH2 turned out to be essential for the initiation and maintenance of global H3K27 trimethylation, a key step in the PcG-mediated transcriptional regulation of genes, we have subsequently examined by DNA-micro...
ORGANISM(S): Homo sapiens 
2007-08-21 | E-TABM-128 | biostudies-arrayexpress
TranscriptomicsMapping and characterization of promoter-binding sites of protein phosphatase 1
Protein phosphatase 1 (PP1) is a Ser/Thr phosphatase that has been implicated in many key cellular functions including transcriptional regulation. Due to its involvement these many processes, it becomes difficult to directly link PP1 to transcriptional regulation on the chromatin level as no direct ...
ORGANISM(S): Homo sapiens 
2015-06-17 | E-GEOD-54170 | biostudies-arrayexpress
TranscriptomicsMapping of EZH2-WT and EZH2-mTP5 binding sites to human promoter regions
To gain genome wide information on the association of EZH2 with promoter regions in HeLa cells, DamID experiments and subsequent analysis by promoter arrays (Affymetrix GeneChip Human Promoter 1.0R ) were performed. The DamID method uses fusions of the bacterial Dam DNA methylase and the protein of ...
ORGANISM(S): Homo sapiens 
2012-11-13 | E-GEOD-39593 | biostudies-arrayexpress
TranscriptomicsExpression profiling of HeLa Tet-off cells expressing Flag-tagged version of WT NIPP1 or PP1 binding mutant of NIPP1.
NIPP1, an established interactor of protein phosphatase 1 (PP1), is implicated in PRC2-mediated regulation of gene expression. Here, we explore whether PP1 associated with NIPP1 is involved in NIPP1-mediated regulation of genes. Therefore, we generated Hela Tet-off (HTO) cell lines that stably and i...
ORGANISM(S): Homo sapiens 
2010-07-19 | E-GEOD-19642 | biostudies-arrayexpress
TranscriptomicsProtein phosphatase PP1-NIPP1 activates mesenchymal genes in HeLa cells
The deletion of the protein phosphatase-1 (PP1) regulator NIPP1 is embryonic lethal during gastrulation, hinting at a key role of PP1-NIPP1 in lineage specification. Consistent with this notion we show here that a mild, stable overexpression of NIPP1 in HeLa cells caused a massive in...
ORGANISM(S): Homo sapiens 
2015-04-04 | E-GEOD-67558 | biostudies-arrayexpress
TranscriptomicsConditional liver NIPP1 KO micro array on total liver RNA
The goal is to investigate genes that are more or less expressed due to the removal of NIPP1 in mouse epithelial liver cells. In this way we hope to unravel the function of NIPP1 in vivo. 4 control and 4 conditional knockout mice were sacrificed. No replicates were performed. Samples were analyzed u...
ORGANISM(S): Mus musculus 
2016-03-27 | E-GEOD-67366 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of Golabi-Ito-Hall derived lymphoblast cells compared to a matched healthy person
A point mutation in the WW domain of PQBP1 that mediates its interaction with SIPP1 causes the Golabi-Ito-Hall (GIH) syndrome, which is associated with severe mental retardation and physical deformations. In this project we compared lymphoblast cell lines from a healthy person and a patient with the...
ORGANISM(S): Homo sapiens 
2011-01-01 | E-GEOD-19724 | biostudies-arrayexpress
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