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MetabolomicsMitochondrial NAD+ Deficiency in Vascular Smooth Muscle Impairs Collagen III Turnover to Trigger Aortic Disease

Aortic disease poses a significant mortality risk in adults, yet many of its underlying factors remain undiscovered. In this study, we identify mitochondrial NAD⁺ deficiency as a causal factor. Metabolomics analysis of 73 surgical aortic specimens revealed impaired NAD⁺ salvage and mitochondrial ...

2024-12-04 | MTBLS8062 | MetaboLights
TranscriptomicsRNA-seq identifies novel lncRNAs involved in vascular smooth muscle cell proliferation
Smooth muscle cell (SMC) phenotypic switching from a contractile to a synthetic state is implicated in diverse vascular pathologies, including neointimal formation. This study was designed to identify lncRNAs that may play a role in vascular pathologies. Primary smooth muscle cells cultured from sur...
ORGANISM(S): Homo sapiens 
2016-05-18 | E-GEOD-69637 | biostudies-arrayexpress
TranscriptomicsStretch-dependent genes in vascular smooth muscle cells
Vascular smooth muscle cells (VSMCs) respond to biomechanical stretch with specific changes in gene expression which govern the phenotype of these cells. The mechanotransducer zyxin is a potential candidate for regulating the expression of such genes. Using microarrays, we compared stretch-induced ...
ORGANISM(S): Mus musculus 
2015-07-01 | E-GEOD-60447 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat A7r5 vascular smooth muscle cells over-expressing glucose transporter
Hypothesis: Overexpression of the GLUT1 facilitative glucose transporter, in A7r5 vascular smooth muscle cells, is sufficient and/or necessary to induce alterations in gene expression which influence apoptosis, growth, and proliferation. Experiment Overall Design: Scientific Approach: A7r5 rat emb...
ORGANISM(S): Rattus norvegicus 
2009-11-07 | E-GEOD-15713 | biostudies-arrayexpress
TranscriptomicsTrophoblast-Induced Changes in C-X-C Motif Chemokine 10 Expression Contribute to Vascular Smooth Muscle Cell Dedifferentiation During Spiral Artery Remodeling
Endothelial cell and vascular smooth muscle cell were cocultured in hanging droplets to form spheroids representing an inverted vessel lumen. Control or conditioned media from an extravillous trophoblast (EVT) cell line was incubated with vascular spheroids for 24 hours. Spheroid RNA was then analyz...
ORGANISM(S): Homo sapiens 
2013-02-17 | E-MTAB-1462 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of endogenous small RNAs from rat vascular smooth muscle cells with and without Angiotensin II stimulation
Angiotensin II (Ang II)-mediated vascular smooth muscle cells (VSMC) dysfunction plays a critical role in cardiovascular diseases. However, the role of microRNAs (miRNAs) in this process is unclear. We used small RNA deep sequencing to profile Ang II-regulated miRNAs in rat VSMC and evaluated their ...
ORGANISM(S): Rattus norvegicus 
2012-03-23 | E-GEOD-35664 | biostudies-arrayexpress
TranscriptomicsPhenotype modulation of VSMCs on patterned substrate
Loss of contractility and acquisition of an epithelial phenotype of vascular smooth muscle cells (VSMCs) are key events in proliferative vascular pathologies such as atherosclerosis and post-angioplastic restenosis. There is no proper cell culture system allowing VSMC differentiation so that it is d...
ORGANISM(S): Rattus norvegicus 
2013-11-14 | E-MTAB-1888 | biostudies-arrayexpress
TranscriptomicsA new approach for the study of lung smooth muscle phenotypes and its application in asthma
We developed a new method for the isolation of bronchial smooth muscle cells. This method is built upon a double fluorescent mouse SMA-GFP;NG2-DsRed and cell sorting. Using this method, we isolated bronchial smooth muscle cells from control and asthmatic C57BL/6J mice for gene profiling. Double fluo...
ORGANISM(S): Mus musculus 
2014-03-13 | E-GEOD-45723 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiling of dedifferentiating smooth muscle cells from mouse aorta and bladder
Smooth muscle cells (SMCs) are important in a number of physiological systems and organs, including the cardiovascular system. The hallmark property of differentiated SMCs is the ability to contract, but contractile SMCs themselves show a range of phenotypes allowing prolonged tonic contraction in v...
ORGANISM(S): Mus musculus 
2016-07-01 | E-MTAB-4841 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human aortic vascular smooth muscle cells treated with IL-17 or untreated
Investigate the effect of recombinant human IL-17A on vascular smooth muscle cells cultured from human aortas. Experiment Overall Design: SMCs from the aortas of three different donors were cultured in M199 media supplemented with 20% FCS and used at passage 3. The cells were either not treated or t...
ORGANISM(S): Homo sapiens 
2008-10-24 | E-GEOD-11367 | biostudies-arrayexpress
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