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ProteomicsProteomics of MCF7 cells (LC-MS/MS Orbitrap Fusion Tribrid)
We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes and associate RNAs by means of an antibody-free and tag-free pull-down approach. We compared riboLace translation estimates with protein levels determined by proteomics in MCF7 c...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD009417 | Pride
Mcf7 Polysome profiling Pol-seq Translation Ribolace Rna-seq 
TranscriptomicsTranslatome and transcriptome profiling of EGF response in HeLa cells
Translatome analysis by sucrose gradient centrifugation of cell lysates followed by microarray profiling of the polysomal and subpolysomal RNA fractions represents a way of both studying translational control networks and better approximating the proteomic representation of cells. It is an establish...
ORGANISM(S): Homo sapiens 
2010-05-27 | E-GEOD-20277 | biostudies-arrayexpress
TranscriptomicsTranslatome and transcriptome profiling of SH-SY5Y cells treated with sublytic doses of staphylococcal alpha-hemolysin
Genome-wide analysis of translation has the potential to provide major contributions in understanding the pathophysiology of infection processes, given the complex interplay between pathogens and host cells. Informations about the translational state of mRNAs or the activity of RNA binding proteins ...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-GEOD-50652 | biostudies-arrayexpress
TranscriptomicsRIP-chip of HuR in MCF7 cells exposed to doxorubicin
The purpose of the study was to identify mRNA bound to HuR in the presence of doxorubicin in MCF7 cells. We collected cytoplasmic RNA from untreated and treated cells and detected differentially expressed genes (DEGs). We also coimmunoprecipitated HuR and IgG (as control) from doxorubicin treated ce...
ORGANISM(S): Homo sapiens 
2011-12-31 | E-GEOD-33055 | biostudies-arrayexpress
TranscriptomicsTranslatome and transcriptome profiling of SH-SY5Y cells treated with staphylococcal alpha-hemolysin oligomers
Genome-wide analysis of translation has the potential to provide major contributions in understanding the pathophysiology of infection processes, given the complex interplay between pathogens and host cells. This study uncovers the reshaping undergoing in the translational control system of the host...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-GEOD-60543 | biostudies-arrayexpress
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