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TranscriptomicsEffect of SOPS and Cryotop vitrification of porcine blastocysts produced in vivo on their transcriptome profile
The objective of this study was therefore to evaluate the impact of simultaneous vitrification of large numbers of embryos with the OC (20 embryos per device) system on gene expression patterns of in vivo-derived blastocysts when compared to the Superfine Open Pulled Straw (SOPS) system (5-7 embryos...
ORGANISM(S): Sus scrofa 
2023-03-06 | E-MTAB-12772 | biostudies-arrayexpress
ProteomicsUnravelling subcellular and ultrastructural changes during vitrification of human spermatozoa: effect of mitochondria-targeted antioxidant and permeable cryoprotectant
The aims of the study were to improve sperm vitrification by using mitochondria-targeted antioxidants (Mito Q), reveal ultrastructural changes in spermatozoa due to the use of permeable cryoprotectants, and report alteration of functional proteins during the sperm vitrification process. For this, ea...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD025939 | Pride
Proteomics Electron microscopy Vitrification Mito q Spermatozoa 
TranscriptomicsVitrification alters fetal development by profound differences during fetal placenta formation
Vitrification is replacing slow freezing as the most popular method for human embryo cryopreservation in clinics world-wide. Several studies demonstrated that cryopreservation alters gene expression of mammalian embryos, but none of them analysed what happen with those embryos that get implanted and...
ORGANISM(S): Oryctolagus cuniculus 
2013-09-26 | E-GEOD-51208 | biostudies-arrayexpress
TranscriptomicsEffect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos
Changes in gene expression induced by the Cryotop vitrification procedure in bovine blastocysts using Agilent EmbryoGENE microarray slides. Bovine in vitro-produced embryos at the blastocyst stage (144 to 156 hours post insemination) were vitrified using the Cryotop system and compared with non-vitr...
ORGANISM(S): Bos indicus 
2015-08-03 | E-GEOD-65953 | biostudies-arrayexpress
ProteomicsVitrification of embryos modifies foetal placenta proteome. LCMSMS
Very limited information about the post-implantatory effects of vitrification has been published till now. In a previous study, we observed that vitrification procedure introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. In addition, a redu...
ORGANISM(S): Oryctolagus cuniculus algirus 
2015-08-11 | PXD001836 | Pride
Cryopreservation  embryos  rabbit  placenta  proteome 
ProteomicsVitrification of embryos modifies foetal placenta proteome. MALDI TOF/TOF
Very limited information about the post-implantatory effects of vitrification has been published till now. In a previous study, we observed that vitrification procedure introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. In addition, a redu...
ORGANISM(S): Oryctolagus cuniculus cuniculus 
2015-07-17 | PXD001840 | Pride
Cryopreservation  embryos  rabbit  placenta  proteome 
ProteomicsCryopreservation of human spermatozoa using penetrating cryoprotectant-free vitrification medium exhibits altered proteomic profile compared to conventional rapid freezing method
Vitrification is not considered as ideal method for cryopreservation of human spermatozoa due to the presence of high concentrations of penetrating cryoprotectants in the vitrification medium. Several studies in the literature suggest that the outcome of semen cryopreservation is superior when cryop...
ORGANISM(S): Homo sapiens (Human) 
2025-07-22 | PXD057601 | Pride
Conventional rapid freezing  mitochondria  motility  acrosome  dna damage  ultrastructural changes 
TranscriptomicsVitrification of pig embryos dysregulates the microRNA transcriptome profile
The aim of this study was to investigate the effects of vitrification on the miRNA transcriptome profile of porcine blastocysts and to compare the effects of the SOPS and Cryotop methods on miRNA profiles. The interactions of the miRNA data with previous gene expression data (Gonzalez-Plaza et al., ...
ORGANISM(S): Sus scrofa 
2024-02-28 | E-MTAB-13814 | biostudies-arrayexpress
GenomicsVitrification preserves follicular transcriptomic dynamics during ex vivo ovulation
Vitrification preserves follicular transcriptomic dynamics during ex vivo ovulation
PRJNA943225 | ENA
TranscriptomicsDirect comparison of effects of slow-freezing and vitrification procedures on rabbit late blastocyst transcriptome
Nowadays there is a need to improve cryopreservation protocols in both human assisted reproduction and domestic animal field. We wanted to compare the transcriptomic changes induced by two commonly used cryopreservation procedures (slow freezing and vitrification) on rabbit late blastocyst before th...
ORGANISM(S): Oryctolagus cuniculus 
2013-09-19 | E-GEOD-50979 | biostudies-arrayexpress
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