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ProteomicsLeaf protein extraction method
We evaluated several approaches for sample processing that are based on popular urea extraction, methanol/chloroform, and phenol methods. Additionally, we examined whether these methods were improved using FASP-based on filter digestion. We found that phenol-FASP and UA-FASP methods enabled the bes...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2018-02-20 | MSV000082080 | MassIVE
ProteomicsArabidopsis Root Protein Extraction Evaluation
We evaluated several approaches for sample processing that are based on popular urea extraction, methanol/chloroform, and phenol methods. Additionally, we examined whether these methods were improved using FASP-based on filter digestion. We found that phenol-FASP and UA-FASP methods enabled the best...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2018-05-15 | MSV000082362 | MassIVE
ProteomicsDRM6 and DLO1 TurboID-based proximity labelling proteomics
TurboID-based proximity labeling of DMR6 and DLO1 identified several candidate interactors, including a putative F-box protein that may contribute to their SCF-dependent turnover.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Nicotiana benthamiana 
2026-03-06 | PXD063821 | Pride
Skp1 Drm6 Salicylic acid Dlo1 Ask1 Proteasome Arabidopsis 
ProteomicsArabidopsis thaliana ASK1 proximity labeling upon Pst DC3000 infection
we used proximity labeling of ASK1, a core SCF adaptor, to profile dynamic changes in SCF-associated networks during Pseudomonas syringae infection. This analysis revealed widespread remodeling of F-box proteins and immune-related regulators, including several previously uncharacterized components.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-05 | PXD063820 | Pride
Skp1 Turboid Ask1 Proteasome Proximity labelling Arabidopsis 
ProteomicsSingle-cell proteomics differentiates Arabidopsis root cell types
Here we explore the feasibility of single-cell proteomics on plant samples. We focus on Arabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-04-17 | MSV000094558 | MassIVE
ProteomicsMouse Placenta Proteome and Phosphoproteome
TMT based quantification of protein abundance and phosphorylation state for developing mouse placenta. Timed-pregnant CD-1 mice were obtained from Charles Rivers Labs and dissected at e7.5 to extract the ectoplacental cones (EPCs) and at e9.5 to obtain the placenta as described by Martin and Cockrof...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-08-24 | MSV000082849 | MassIVE
ProteomicsIdentification of the Kinase phosphorylation sites on AtGPA1 in presence of GDP
Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., 2018, J. Biol. Chem 293(13): 4752-4766). Among them, we here cho...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2019-06-12 | MSV000083963 | MassIVE
ProteomicsBIN2 and RAPTOR1B mutants phosphoproteomic analysis
Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086460 | MassIVE
ProteomicsArabidopsis thaliana whole-proteome BIN2 kinase assay
Finely ground leaf tissue of wild-type columbia-0 ecotype was used. Protein extraction was performed in buffer-saturated phenol and high sucrose buffer. Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase. Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086462 | MassIVE
ProteomicsQuantitative proteomics reveals extensive lysine ubiquitination in Arabidopsis thaliana
In this study, we describe an antibody-based approach to enrich ubiquitinated peptides from vegetative tissues for detection via peptide mass spectrometry. This enrichment method can be coupled with isobaric labeling to enable quantification from up to 18-multiplexed samples. This approach identifie...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-07-31 | MSV000095496 | MassIVE
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